Page 96 - GPD-2-1

Gene & Protein in Disease

ORIGINAL RESEARCH ARTICLE
Underexpression of SCN7A is associated with

poor prognosis in lung adenocarcinoma

Hehui Lv 1,2† , Hongyuan Song 1,2† , Zhouping Qin 1,2† , Rongchun Xing , and
1,2
Yulian Chen *
1,2
1 First Hospital of Yichang, Hubei, China
2 People’s Hospital of China Three Gorges University, Hubei, China

Abstract
Lung cancer is one of the most common malignancies and the leading cause
of cancer-related deaths worldwide. Elucidating the mechanism behind the
development of lung cancer at a molecular level could reveal new biomarkers and
clinical therapeutic targets. A growing body of evidence has shown an association
between ion channel-related genes and the progression of various diseases,
including cancer. However, the association is not well-understood. In this study,
we identified ion channel-related genes differentially expressed between cancer
and normal lung tissue cells. Data were extracted from GSE31552, GSE33532, and
GSE103512 lung adenocarcinoma (LUAD) datasets, and the prognostic value of the
differentially expressed genes between LUAD and normal lung tissue was evaluated
using data in The Cancer Genome Atlas. Only sodium voltage-gated channel
alpha subunit 7 (SCN7A) was found to be significantly correlated to prognosis. The
expression of SCN7A in LUAD was assessed further by immunological analysis. We
† These authors have contributed also constructed a competing endogenous RNA network of SCN7A. Further analyses
equally to this work.
revealed that the underexpression of SCN7A predicted a poor prognosis in LUAD,
*Corresponding author: with a strong correlation between SCN7A expression and immune cell infiltration as
Yulian Chen
(chenyulian213586@163.com) well as immune checkpoint expression. We found that SCN7A is potentially regulated
via the OTUD6B-AS1-miR-21-5p-SCN7A axis in LUAD cells and proved that SCN7A
Citation: Lv H, Song H, Qin Z, inhibits the proliferation and migration of lung cancer cells in vitro. Taken together,
et al., 2023, Underexpression of
SCN7A is associated with poor SCN7A, as an independent prognostic factor, is a promising diagnostic biomarker for
prognosis in lung adenocarcinoma. LUAD, and the OTUD6B-AS1-miR-21-5p-SCN7A axis is a potential regulatory network
Gene Protein Dis. 2(1):363. of SCN7A expression.
https://doi.org/10.36922/gpd.363
Received: February 8, 2023
Accepted: March 16, 2023 Keywords: Lung adenocarcinoma; Gene Expression Omnibus; The Cancer Genome Atlas;
Published Online: March 27, 2023 Immune infiltration; Competing endogenous RNA; Prognosis
Copyright: © 2023 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, 1. Introduction
and reproduction in any medium,
provided the original work is Lung cancer is the leading cause of cancer-related mortality worldwide, accounting for
properly cited. nearly 2 million reported cases and 1.76 million deaths in 2020 . Non-small cell lung
[1]
Publisher’s Note: AccScience cancer accounts for 80% of all lung malignancies, and the overall 5-year survival rate of
Publishing remains neutral with lung adenocarcinoma (LUAD) is 5% [2,3] . Despite the numerous treatments available, the
regard to jurisdictional claims in
published maps and institutional prognosis of LUAD remains unsatisfactory. Due to the fact that early-stage lung cancer is
affiliations. usually asymptomatic, the majority of patients with lung cancer are diagnosed at a more

Volume 2 Issue 1 (2023) 1 https://doi.org/10.36922/gpd.363