Page 84 - GPD-2-4

Gene & Protein in Disease

ORIGINAL RESEARCH ARTICLE
Association of CCR2 gene polymorphism with

chronic kidney disease in the North Indian
population

1
Aseem Yadav , Saliha Rizvi *, Devendra Kumar , Syed Tasleem Raza ,
3
1
2
Jalees Fatima , Farheen Khan , and Ajay Mishra 1
1
2
1 Department of Medicine, Era’s Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India
2 Department of Biotechnology, Era University, Lucknow, Uttar Pradesh, India
3 Department of Biochemistry, Era’s Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India

Abstract
Chronic kidney disease (CKD), characterized by decreased renal function, is associated
with an underlying elevated renal inflammatory state. The chemokine receptor
2 (CCR2) mediates leukocyte chemoattraction in the initiation and amplification
phases of renal inflammation. In this study, which involved 62 patients and
62 controls, we aim to elucidate the association between the CCR2 G190A (rs1799864)
polymorphism and CKD. Polymerase chain reaction-restriction fragment length
polymorphism technique-based single-nucleotide polymorphism genotyping of
the CCR2 gene (rs1799864) was used to assess the allele and genotype frequencies
of CCR2. The Chi-square test was used to assess the potential association between
G190A polymorphism and CKD, including its stages. The presence of the CCR2 G190A
polymorphism was significantly associated with CKD. The results unveiled a significant
difference in the genotype and allele frequency distribution of CCR2 G190A in CKD
patients and control subjects. CCR2 GA genotype (P = 0.003) and A allele (P = 0.007;
*Corresponding author:
Saliha Rizvi odds ratio [OR] = 0.40; 95% confidence interval [CI] = 0.20 – 0.80) were found to be
(saliha.rizvi@erauniversity.in) significantly associated with CKD. A significant association was identified between
Citation: Yadav A, Rizvi S, genotype and stage of CKD, with the GA genotype more common among end-stage
Kumar D, et al., 2023, Association renal disease (ESRD) (stage 5) and the GG genotype among non-ESRD (stages 1 – 4)
of CCR2 gene polymorphism with patients (P < 0.001). The polymorphism was significantly associated with deteriorating
chronic kidney disease in the North
Indian population. Gene Protein Dis. renal function, as evidenced by elevated levels of serum urea, serum creatinine, and spot
https://doi.org/10.36922/gpd.2253 urine, alongside a decrease in serum calcium and estimated glomerular filtration rate.
The study revealed that the GA genotype of CCR2 is associated with CKD, ESRD, severe
Received: November 15, 2023
Accepted: January 5, 2024 albuminuria, and renal dysfunction. However, no association was observed between
Published Online: January 10, 2024 the CCR2 gene polymorphism and the causes of CKD in the North Indian population.
Copyright: © 2024 Author(s).
This is an Open-Access article
distributed under the terms of the Keywords: Chronic kidney disease; End-stage renal disease; Chemokine receptor-2;
Creative Commons Attribution Single-nucleotide polymorphism; Polymerase chain reaction-restriction fragment length
License, permitting distribution, polymorphism
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with 1. Introduction
regard to jurisdictional claims in [1]
published maps and institutional Chronic kidney disease (CKD) is a leading public health problem worldwide and affects
affiliations. between 8% and 16% of the global population. However, evidence suggests that it is

Volume 2 Issue 4 (2023) 1 https://doi.org/10.36922/gpd.2253