Page 441 - IJB-10-1

International

Journal of Bioprinting

RESEARCH ARTICLE
Low-temperature deposition 3D printing biotin-

doped PLGA/β-TCP scaffold for repair of bone
defects in osteonecrosis of femoral head

Peng Xue , Xiaoxue Tan , Hongzhong Xi , Hao Chen , Shuai He ,
1,2
3
1,2
1,2
1,2
Guangquan Sun , Changyuan Gu , Xiaohong Jiang , Bin Du 1,2* , and Xin Liu 1,2*
1,2
1,2
3
1 Department of Orthopedics, The Affiliated Hospital of Nanjing University of Chinese Medicine,
Nanjing 210029, China
2 Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu 210029, China
3 International Chinese-Belorussian Scientific Laboratory on Vacuum-Plasma Technology, Nanjing
University of Science and Technology, Nanjing 210094, China
(This article belongs to the Special Issue: Biomedical Application of 3D Bioprinting)
Abstract
The removal of necrotic bone and implantation of bone repair materials is an effective
treatment for osteonecrosis of the femoral head (ONFH)-type bone defects, but there
are currently no clinically applicable bone repair materials. In this study, a biotin-
doped bone repair scaffold was created using low-temperature deposition (LTD)
three-dimensional (3D) printing technology, and its ability to repair bone defects in
ONFH was evaluated. The scaffold was characterized in vitro, and its cytotoxicity and
*Corresponding authors: osteogenic capacity were assessed by co-culturing the scaffold with rat bone marrow
Xin Liu mesenchymal stem cells. The scaffolds were implanted in an animal model of ONFH-
(liuxinsurg@163.com) type bone defects, and the effect of scaffolds on promoting bone repair was evaluated
Bin Du
(fsyy00608@njucm.edu.cn) by means of radiology and histopathology. LTD 3D-printed biotin-doped scaffolds
showed cancellous bone-like structures without inducing cytotoxicity, whereas
Citation: Xue P, Tan X, Xi H, et al. high-biotin β-TCP scaffolds (HBPT; containing 2% biotin) promoted osteogenic
Low-temperature deposition 3D
printing biotin-doped PLGA/β-TCP differentiation more effectively. Experiments on animals revealed that the effect of
scaffold for repair of bone defects in HBPT on bone repair was significantly superior to that of other groups. The in vivo
osteonecrosis of femoral head. Int J biocompatibility of HBPT was confirmed by blood analysis and hematoxylin and eosin
Bioprint. 2024;10(1):1152.
doi: 10.36922/ijb.1152 staining of the main organs. In conclusion, biotin-doped scaffolds can be used to treat
ONFH-type bone defects by virtue of their ability in promoting bone regeneration.
Received: June 26, 2023
Accepted: August 10, 2023
Published Online: September 12,
2023 Keywords: Biotin; Low-temperature deposition; Bone repair; Osteonecrosis of the
femoral head; Bone tissue engineering scaffold
Copyright: © 2023 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, 1. Introduction
and reproduction in any medium,
provided the original work is Osteonecrosis of the femoral head (ONFH) is a common orthopedic condition that,
properly cited.
if left untreated, can contribute to hip disability by causing hip pain and dysfunction
Publisher’s Note: AccScience as the disease progresses. Removal of necrotic bone and implantation of bone
1-3
Publishing remains neutral with
4
regard to jurisdictional claims in repair materials are standard clinical procedures. Allogeneic bone or bioceramics
published maps and institutional are currently utilized as bone repair materials, but both have numerous drawbacks.
5-7
affiliations. Homogeneous allogeneic bone is a scarce and costly material; in addition to the lack

Volume 10 Issue 1 (2024) 433 https://doi.org/10.36922/ijb.1152