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Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

            the native tissue architecture. 3D printing of scaffolds   Conflict of Interest and Funding
            for  tissue engineering  and  3D  Bioprinting  are rela-
            tively new technologies which  have the potential  to   No conflict of interest was reported by the authors.
            meet this requirement. In this study, E-jetting process   References
            is studied in detail and the effect of various parame-
            ters on the printed fibre diameter analysed, pertaining   1.   Hutmacher D  W, 2001, Scaffold design and fabrication
            to  PCL biomaterial. The relationship  between  these   technologies  for engineering  tissues  —  state  of the  art
            parameters,  in  combination, is complex. Hence, a      and future perspectives. Journal of Biomaterials Science,
                                                                    Polymer Edition, vol.12(1): 107–124.
            detailed  parametric study has  to  be  performed for   http://dx.doi.org/10.1163/156856201744489
            finding the optimum parameters of E-jetting to print   2.   Hutmacher  D  W,  Schantz  T,  Zein  I,  et  al.,  2001,  Me-
            stable, regular, continuous fibres and hence scaffolds   chanical properties and cell cultural response of polyca-
            with good structural and spatial properties. In fact, the   prolactone  scaffolds  designed  and  fabricated  via  fused
            greatest advantage of this technology is the patterning   deposition  modelling.  Journal  of  Biomedical  Materials
            and orientation  of  fibres in a controlled manner in   Research, vol.55(2): 203–216.
            desired  architecture. The complex  geometries like     http://dx.doi.org/10.1002/1097-4636(200105)55:23.0.C
                                                                    O;2-7
            semi-lunar and  spiral shaped  scaffolds are printed   3.   Cukierman E, Pankov R, Stevens D R, et al., 2001, Tak-
            using  this  technique, which will be very useful  for   ing cell-matrix adhesions to the third dimension. Science,
            certain  complex  soft  tissues  in  body  like  the  knee   vol.294(5547): 1708–1712.
            meniscus or tendon. These different complex shapes      http://dx.doi.org/10.1126/science.1064829
            are  also  expected  to  influence the  cell proliferation   4.   Cukierman E, Pankov R and Yamada K  M, 2002, Cell
            positively, in terms of better cell migration within the   interactions  with  three-dimensional  matrices.  Current
                                                                    Opinion in Cell Biology, vol.14(5): 633–640.
            scaffold  and  formation of cell-cell junctions, which   http://dx.doi.org/10.1016/S0955-0674(02)00364-2
            has to be validated by future in vitro studies. Devel-  5.   Edelman D B and Keefer E W, 2005, A cultural renais-
            opment of the field of tissue engineering and success-  sance: In vitro cell biology embraces three-dimensional
            ful clinical translation of this technology depends on   context. Experimental Neurology, vol.192(1): 1–6.
            how closely the engineered tissue biomimic the native   http://dx.doi.org/10.1016/j.expneurol.2004.10.005
            tissue  architecture.  Both  3D  printing  of scaffolds  for   6.   Sachlos E and Czernuszka J T, 2003, Making tissue en-
                                                                    gineering  scaffold  work:  Review  on  the application of
            tissue  engineering  and 3D  bioprinting  are  relatively   SFF technology to the production of tissue engineering
            new technologies which have the potential to meet this   scaffolds. European Cells  and  Materials, vol.5(1): 29–
            requirement. In this study, E-jetting process is studied   40.
            in  detail  and  the  effect  of  various  parameters  on the   7.   Whitesides  G  M,  Ostuni  E,  Takayama  S,  et  al.,  2001,
            printed fibre  diameter  analyzed,  pertaining  to  PCL   Soft  lithography  in  biology  and  biochemistry.  Annual
            biomaterial.  The relationship  between these  parame-  Review of Biomedical Engineering, vol.3(1): 335–373.
                                                                    http://dx.doi.org/10.1146/annurev.bioeng.3.1.335
            ters, in  combination, is  complex.  Hence,  a detailed   8.   Walker G M, Zeringue H C and Beebe D  J, 2004, Mi-
            parametric study  has to be  performed for finding the   croenvironment design considerations for cellular scale
            optimum parameters of E-jetting process to print sta-   studies. Lab on a Chip, vol.4(2): 91–97.
            ble,  regular,  continuous  fibres  and  hence scaffolds   http://dx.doi.org/10.1039/b311214d
            with good structural and spatial properties. In fact, the   9.   Khademhosseini A, Langer R, Borenstein J, et al., 2006,
            greatest advantage of this technology is the patterning   Microscale technologies for tissue engineering and biol-
                                                                    ogy. Proceedings of the National Academy of Sciences of
            and orientation of fibres in a controlled manner in de-  the United States of America, vol.103(8): 2480–2487.
            sired architecture. The complex geometries like semi-   http://dx.doi.org/10.1073/pnas.0507681102
            lunar and spiral shaped scaffolds are printed using this   10.  Lannutti J, Reneker D, Ma T, et al., 2007, Electrospin-
            technique, which will be very useful for certain com-   ning for tissue engineering scaffolds. Materials Science
            plex soft  tissues  in  body  like  the  knee  meniscus  or   and Engineering: C, vol.27(3): 504–509.
            tendon.  These  different  complex  shapes  are  also  ex-  http://dx.doi.org/10.1016/j.msec.2006.05.019
            pected to influence the cell  proliferation  positively,  in   11.  Agarwal S, Wendorff J H and Greiner A, 2009, Progress
                                                                    in the field of electrospinning for tissue engineering ap-
            terms of better cell migration within the scaffold  and   plications.  Advanced  Materials,  vol.21(32–33):  3343–
            formation  of cell-cell junctions, which has to be vali-  3351.
            dated by future in vitro studies.                       http://dx.doi.org/10.1002/adma.200803092

            70                          International Journal of Bioprinting (2016)–Volume 2, Issue 1
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