Page 466 - IJB-10-2

International

Journal of Bioprinting

RESEARCH ARTICLE
Bioprinting with adipose stem cells and hydrogel

modified with bioactive glass

Krishna C.R. Kolan , Apurv Saxena , Bradley A. Bromet , Lesa B. Steen ,
1
3
4
2
August T. Bindbeutel , Julie A. Semon *, Delbert E. Day , and Ming C. Leu 1
3
4
1
1 Department of Mechanical and Aerospace Engineering, Missouri University of Science and
Technology, Rolla, Missouri, United States of America
2 Department of Chemistry, Missouri University of Science and Technology, Rolla, Missouri, United
States of America
3
Department of Biological Sciences, Missouri University of Science and Technology, Rolla,
Missouri, United States of America
4 Department of Material Science and Engineering, Missouri University of Science and Technology,
Rolla, Missouri, United States of America
Abstract

Bioprinting research is focused on utilizing growth factors and multiple cell types
to create clinically relevant three-dimensional (3D) tissue models using hydrogels.
Rheological and biological challenges are two main factors that limit the creation
of extrudable bioactive hydrogels. In this study, we investigate incorporation of fast
dissolving and bioactive borate glass in different weight to volume percentages
(0.075 to 0.6%) to alginate-gelatin (1:1) hydrogel to improve rheological properties
and enable bioprinting with bioactive glass. The addition of glass improved the
stiffness of the hydrogel. Human adipose-derived mesenchymal stem cells (ASCs)
were uniformly mixed in this bioink at 1 × 10 cells/mL concentration, and spheroid
6
*Corresponding author:
Julie A. Semon specimens were cultured in both static and dynamic culture conditions. Grid-
3
(semonja@mst.edu) shaped scaffolds measuring ~18 × 18 × 1 mm were fabricated with the viable glass
concentrations, and ASC viability was evaluated using Live/Dead assay. Despite
Citation: Kolan KCR, Saxena A,
Bromet BA, et al. Bioprinting with immediate toxicity, an increased viability after 7 days with 0.15 w/v % or less borate
adipose stem cells and hydrogel glass content demonstrated the potential in utilizing highly resorbable calcium-
modified with bioactive glass. releasing biomaterials such as bioactive glasses to modify hydrogels suitable for
Int J Bioprint. 2024;10(2):2057.
doi: 10.36922/ijb.2057 bioprinting cellularized 3D structures.
Received: October 18, 2023
Accepted: January 31, 2024 Keywords: Bioink; Bioactive glass; Ceramics; Adipose stem cells; Alginate; Gelatin
Published Online: March 1, 2024
Copyright: © 2024 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution 1. Introduction
License, permitting distribution,
and reproduction in any medium, Extrusion-based bioprinting is gaining in popularity because its versatility enables
1
provided the original work is the fabrication of materials over a wide range of viscosities. A majority of the current
properly cited. generation commercial bioprinters are all based on extrusion three-dimensional (3D)
2
Publisher’s Note: AccScience printing techniques. The success of extrusion-based bioprinting depends largely on
Publishing remains neutral with the proper selection of an extrudable hydrogel precursor and the proper selection of
regard to jurisdictional claims in
published maps and institutional cell type. Hydrogels such as alginate, chitosan, hyaluronic acid, gelatin, collagen, fibrin,
®
affiliations. Matrigel, Pluronic F127, and polyethylene glycol are commonly used to encapsulate

Volume 10 Issue 2 (2024) 458 doi. 10.36922/ijb.2057