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3D bioprinting of stem cells and polymer/bioactive glass composite scaffolds for bone tissue engineering

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Printing process parameters were identified for the http://dx.doi.org/10.1007/s10856-011-4236-4
3
composite and bio-ink to fabricate a (10×10×1) mm 6. Kolan K C R, Leu M C, Hilmas G E, et al., 2012, Effect
ASC-laden scaffold with pore sizes ranging from 100 of material, process parameters, and simulated body
to 300 µm suitable for bone tissue engineering. In fluids on mechanical properties of 13–93 bioactive glass
comparison to the conventional melt-deposition extru- porous constructs made by selective laser sintering.
sion 3D bioprinting, the degradation of polymer/bioa- Journal of Mechanical Behaviour of Biomedical Mate-
ctive glass scaffolds showed a c ontrolled release rials, vol.13: 14–24.
of bioactive glass with ~23% weight loss in two http://dx.doi.org/10.1016/j.jmbbm.2012.04.001
weeks. Formation of hydroxyapatite-like crystals on 7. Bartolo P, Kruth J P, Silva J, et al., 2012, Biomedical
the surface of the scaffold after soaking in culture me- production of implants by additive electro-chemical and
dia for up to two weeks shows the strong bioactivity physical processes. CIRP Annals — Manufacturing
of the fabricated composite scaffold and its high po- Technology, vol.61(2): 635–655.
tential for bone repair. The live/dead assay showed http://dx.doi.org/10.1016/j.cirp.2012.05.005
more than 60% viable ASCs on the scaffold after 1 8. Temple J P, Hutton D L, Hung B P , et al., 2014,
Engineering anatomically shaped vascularized bone
week of incubation, with minimal negative effects grafts with hASCs and 3D-printed PCL scaffolds. Journal
from chloroform evaporation on the cells. The results of Biomedical Materials Research Part A, vol.102(12):
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vent-based extrusion 3D bioprinting process to fabri- http://dx.doi.org/10.1002/jbm.a.35107
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Conflict of Interest and Funding vol.7(6): 2355–2373.
http://dx.doi.org/10.1016/j.actbio.2011.03.016
No conflict of interest was reported by the authors. 10. Liu X and Ma P X, 2004, Polymeric scaffolds for bone
tissue engineering. Annals Biomedical Engineering,
Acknowledgment vol.32(3): 477–486.
http://dx.doi.org/10.1023/B:ABME.0000017544.36001.8e
The glass used in this study was provided by MO-SCI 11. Bose S, Vahabzadeh S and Bandyopadhyay A, 2013,
Corporation, Rolla, Missouri, USA. The authors thank Bone tissue engineering using 3D printing. Materials
Mariahe Long for her assistance during experiments Today, vol.16(12): 496–504.
and imaging. http://dx.doi.org/10.1016/j.mattod.2013.11.017
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