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International Journal of Bioprinting Design and optimization of 3DP bioscaffolds

and culture medium at a volume ratio of 1:9. The scaffolds 3.5. Optimization of channeled scaffold
were placed in a 48-well plate, washed with PBS, and then Scaffolds featuring multiple channels play an essential role in
immersed in an adequate amount of CCK-8 solution to tissue engineering as they are able to guide cell growth (such
cover the samples fully. The plate was incubated in a CO as nerve cells used for spinal cord injury repair) or facilitate
2
incubator for 2 h. After incubation, the CCK-8 solution nutrient transportation necessary for vascularization and
14,15
was transferred to a new plate, and the absorbance of tissue regeneration. A two-step optimization strategy
based on the multi-physics model was proposed for
the solution was measured using an ELISA reader at optimizing the structural parameters of channeled scaffolds
a wavelength of 450 nm. The cells were enumerated so that the cell quantity and the averaged cell density were
by converting the absorbance readings using standard optimal simultaneously, while keeping hypoxia lower than
equation. The cell proliferation data were primarily used to the threshold C <14 μM. The first step of the optimization
20
validate the model. method was to find the optimal volume fraction of a

Figure 5. Flowchart of the “two-step optimization method” for optimizing channeled scaffolds. The first step is to find an appropriate volume fraction of
a single channel in the scaffold to ensure that the cell quantity and average cell density are optimal simultaneously. In the second step, the volume of the
single channel is spread evenly to the scaffold to form a multi-channeled structure. Abbreviation: DLP, digital light processing.

Volume 10 Issue 3 (2024) 286 doi: 10.36922/ijb.1838