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Table 1. Summary of different bioprinting and bioassembly technologies
Bioprinting Techniques Materials Resolution Advantages Reference
Good resolution, Ultrafine droplets
Material Hydrogel: e.g. Alginate, collagen, <10 µm to 200 µm diameter, availability, [18–21]
Jetting fibrin, agarose, gelatin methacryloyl, Droplet volume of 1 pL to Precise deposition of materials
polyethylene glycol 7000 pL High speed printing of droplet
Hydrogel: e.g. Alginate, collagen, Relatively good resolution,
Material Extrusion fibrin, agarose, gelatin methacryloyl, 15–400 µm Easy to implement, [29–38]
polyethylene glycol Wide range of hydrogel materials,
Potential for multi-material bioprinting
Good resolution, Fast printing speed,
Vat Polymerization Light sensitive photopolymers 5–100 µm Nozzle-free, [60,61,67]
Printing (e.g. GelMA, PEGDA) Potentially free from support-structure
Bioassembly or Bio- Depends on the size of Direct cell manipulation, Scaffold-free [69–71]
placer Microtissues (cell spheroids, cell sheet) microtissues Multi-cellular construct for complex
tissue
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3. Murphy S V, Atala A, 2014, 3D bioprinting of tissues and 12. Fang Y, Frampton J P, S Raghavan S, et al., 2012, Rapid
organs. Nat Biotechnol, 2014. 32(8): 773–785. http://dx.doi. generation of multiplexed cell cocultures using acoustic
org/10.1038/nbt.2958 droplet ejection followed by aqueous two-phase exclusion
4. Mandrycky C, Wang C, Kim K, et al., 2016, 3D bioprinting patterning. Tissue Eng Part C Methods, 18(9): 647–657.
for engineering complex tissues. Biotechnol Adv, 34(4): http://dx.doi.org/10.1089/ten.tec.2011.0709
422–434. http://dx.doi.org/10.1016/j.biotechadv.2015.12.011 13. Eagles P A , Qureshi A N, Jayasinghe S N, 2006,
5. Zhang Y S, Yue K, Aleman J, et al., 2017, 3D bioprinting for Electrohydrodynamic jetting of mouse neuronal cells.
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thermoresponsive hydrogels for next generation tissue Electrohydrodynamic jet processing: An advanced electric-
engineering: A review. Macromol Mater Eng, 302: 1600266. field-driven jetting phenomenon for processing living
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