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Figure 5. Live/dead staining of mouse myoblast cells encapsulated in peptide hydrogels, 4 mg/mL CH-01 and 3 mg/mL CH-02, and 30
mg/mL alginate-gelatin (1:1), for different time points. Alginate-Gelatin used as positive control (B, E, H), CH-01 (C, F, I), and CH-02 (D,
G, J) at day 2, 4 and 8, respectively. Scale bars 100 μm.
Figure 6. Overlaid confocal fluorescence images of mouse myoblast cells encapsulated in peptide (4 mg/mL CH-01 and 3 mg/mL; CH-
02) and alginate-gelatin (1:1) hydrogels. The encapsulated cells were cultured for different days and finally analyzed using fluorescence
confocal microscopy (Nucleus shown in blue, F-actin shown in red and vinculin in green). (A) Mouse myoblast cells cultured on tissue
culture plate (TCP). Alginate-gelatin (B, C, D), CH-01 (E, F, G), and CH-02 (H, I, J) at day 2, 4, and 8, respectively. Scale bar is 20 µm.
International Journal of Bioprinting (2018)–Volume 4, Issue 2 7
