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A continuous net-like eutectic structure enhances the corrosion resistance of Mg alloys
           the AZ61-Ti extracts exhibited improved cell  viability   increase of Ti, the microhardness gradually increased to
           compared with AZ61 as the cell density (number of cells)   90.1 ± 4.0 HV for AZ61-0.25Ti, 95.2 ± 3.4 HV for AZ61-
           was  enhanced.  Specifically,  AZ61-0.5Ti  exhibited  the   0.5Ti, 96.3 ± 3.1 HV for AZ61-0.75Ti, and 97.8 ± 4.3 HV
           best cell viability. There were few apoptotic  cells (red   for AZ61-1.0Ti, respectively (Figure 9B).
           fluorescence in the nuclei) in each group for all culture
           periods.  Moreover, cells  exhibited  similar  shape  after   4. Discussion
           culturing for 24 h and 72 h. Clearly, AZ61-0.5Ti exhibited
           improved biocompatibility as compared with AZ61. It was   4.1 Microstructure and Mechanical
           believed  that  the enhanced  corrosion resistance  should   Properties
           be responsible for the improved biocompatibility . For
                                                    [26]
           AZ61-0.5Ti, the enhanced corrosion resistance resulted in   In the present study, the incorporation  of moderate  Ti
           a reduced degradation rate, thus decreasing the corrosion   induced the formation of continuous net-like eutectic
           products, which might alleviate the cytotoxicity .  α phase. It was believed  that  Ti,  which had low solid
                                                   [27]
                                                               solubility  in  Mg,  would  be  first  rejected  into  the
           3.4 Mechanical Properties                           interdendritic liquid phase during the solidification of the
                                                                         [28]
           The compressive strength and microhardness of AZ61-  molten pool . From the perspective of thermodynamics,
           Ti are displayed in Figure 9. AZ61 exhibited a relatively   Ti in the interdendritic liquid would catch the Al atoms
           low compressive strength of 110.4 ± 15.2 MPa. With Ti   in Mg solute to form the second phase due to the high
                                                                                     [15]
           gradually increasing to 0.5 wt%, the compressive strength   affinity between Ti and Al . Thus, the diffusivity of Al
           was gradually improved to 175.6±21.3 MPa. However,   would be enhanced during the solidification, resulting in
           with Ti content further increasing to 0.75 wt% and 1.0   an increased Al content near the eutectic location. With
           wt%, the compressive strength was decreased to 145.3 ±   the liquid phase cooling to the eutectic temperature and
           30.9 MPa and 123.9 ± 34.7 MPa, respectively. AZ61 had   the Al content close to eutectic composition, the eutectic
           a relatively low microhardness of 84.8 ± 3.7 HV. With the   reaction would occur to form the eutectic phases.


                           A                                 B


















                                            C


















           Figure 7. (A) pH of the extracts; (B) Mg and Zn concentration in the extracts; (C) relative viability of MG63 cells culture in AZ61-Ti
           extracts.

           56                          International Journal of Bioprinting (2019)–Volume 5, Issue 2
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