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International Journal of Bioprinting                                DIW of concave hydroxyapatite scaffolds




            stability, as it is more difficult for the CDHA nanocrystals   based structures, is displayed in Figure 3. The printed OP
            to interlock during the hardening process.         structure has a total porosity of 20.17%, relatively close
                                                               to the designed structure in the G-code (i.e., 20%). The
            3.2. Physicochemical characterization of           total porosity was slightly increased in the TPMS-based
            the scaffolds                                      structures: 24.51% for G, 26.28% for D, and 24.65% for S.
            Porosity analysis for a 5.70 cm diameter and 4.29 cm height   Similar trends were observed for the closed porosity of the
            region,  corresponding  to  2.66  ×  2  unit  cells  in  TPMS-  TPMS-based scaffolds: 2.63% for OP, 3.35% for G, 3.65%
































































            Figure 3. Porosity analysis by computed microtomography of the 3D-printed calcium-deficient hydroxyapatite (CDHA) scaffolds with either a typical
            orthogonal pattern (OP) or three triply periodic minimal surface (TPMS)-based structures (gyroid [G], diamond [D], and Schwarz [S]) printed with 20%
            nominal porosity: (a) total and closed porosity; (b) connectivity; (c) binary segmentation of different sections; and (d) trabecular separation distribution.
            Scale bars: 1 mm (c, top); 500 µm (c, bottom).

            Volume 10 Issue 6 (2024)                       232                                doi: 10.36922/ijb.3805
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