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RESEARCH ARTICLE

3D-printed HA15-loaded β-Tricalcium Phosphate/

Poly (Lactic-co-glycolic acid) Bone Tissue Scaffold

Promotes Bone Regeneration in Rabbit Radial Defects

Chuanchuan Zheng , Shokouh Attarilar , Kai Li , Chong Wang , Jia Liu *, Liqiang Wang ,
5
1†
1
3†
4
2†
Junlin Yang , Yujin Tang *
1
2
1 Department of Orthopedics, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, China
2 Department of Pediatric Orthopaedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,
Shanghai, 200092, China
3 Academy of Orthopedics, Guangdong Province, Guangdong Provincial Key Laboratory of Bone and Joint Degeneration
Diseases, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510000, China.
4 School of Mechanical Engineering, Dongguan University of Technology, Dongguan, Guangdong, 523808, China
5 State Key Laboratory of Metal Matrix Composites, School of Material Science and Engineering, Shanghai Jiao Tong
University, Shanghai, 200240, China
† These authors contributed equally to this work.
Abstract: In this study, a β-tricalcium phosphate (β-TCP)/poly (lactic-co-glycolic acid) (PLGA) bone tissue scaffold was
loaded with osteogenesis-promoting drug HA15 and constructed by three-dimensional (3D) printing technology. This drug
delivery system with favorable biomechanical properties, bone conduction function, and local release of osteogenic drugs
could provide the basis for the treatment of bone defects. The biomechanical properties of the scaffold were investigated
by compressive testing, showing comparable biomechanical properties with cancellous bone tissue. Furthermore, the
microstructure, pore morphology, and condition were studied. Moreover, the drug release concentration, the effect of anti-
tuberculosis drugs in vitro and in rabbit radial defects, and the ability of the scaffold to repair the defects were studied. The
results show that the scaffold loaded with HA15 can promote cell differentiation into osteoblasts in vitro, targeting HSPA5.
The micro-computed tomography scans showed that after 12 weeks of scaffold implantation, the defect of the rabbit radius was
repaired and the peripheral blood vessels were regenerated. Thus, HA15 can target HSPA5 to inhibit endoplasmic reticulum
stress which finally leads to promotion of osteogenesis, bone regeneration, and angiogenesis in the rabbit bone defect model.
Overall, the 3D-printed β-TCP/PLGA-loaded HA15 bone tissue scaffold can be used as a substitute material for the treatment
of bone defects because of its unique biomechanical properties and bone conductivity.
Keywords: Three-dimensional printing; β-tricalcium phosphate; HA15; Endoplasmic reticulum stress; Bone defect

*Correspondence to: Yujin Tang, Department of Orthopedics, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise,
Guangxi, 533000, China; tangyujin1967@163.com. Jia Liu, Department of Orthopedics, Affiliated Hospital of Youjiang Medical University for
Nationalities, Baise, Guangxi, 533000, China; liujia0111@live.cn
Received: October 30, 2020; Accepted: November 23, 2020; Published Online: January 20, 2021

Citation: Zheng C, Attarilar S, Li K, et al., 2021, 3D-printed HA15-loaded β-Tricalcium Phosphate/Poly (Lactic-co-glycolic
acid) Bone Tissue Scaffold Promotes Bone Regeneration in Rabbit Radial Defects. Int J Bioprint, 7(1):317.http://doi.
org/10.18063/ijb.v7i1.317

1. Introduction including bone atrophy, trauma, benign and malignant
tumors, and periodontal disease . In this regard, an
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Bone defects, which are of prominent importance, refer autologous bone graft is a unique standard of the hard tissue
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to a range of injuries that happen due to different reasons, transplantation treatment, especially for bone defects .
© 2021 Zheng, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International
License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the
original work is properly cited.
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