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A Dual-Sensitive Hydrogel for 3D Printing
4. Conclusions 4. Bedell ML, Navara AM, Du Y, et al., 2020, Polymeric

In summary, a thermo-sensitive and photo-sensitive Systems for Bioprinting. Chem Rev, 120:10744–92.
hydrogel based on the triblock copolymers PLGA-PEG- 5. Li X, Liu B, Pei B, et al., 2020, Inkjet Bioprinting of
PLGA was developed for 3D printing. Aqueous solutions Biomaterials. Chem Rev, 120:10793–833.
of the copolymers exhibited thermo-induced sol-gel 6. Valot L, Martinez J, Mehdi A, et al., 2019, Chemical Insights
transition accompanied by the increase of the viscosity. Into Bioinks for 3D Printing. Chem Soc Rev, 48:4049–86.
The resulted hydrogels showed excellent shear-thinning https://doi.org/10.1039/c7cs00718c
properties and fast elastic recovery properties, which 7. Murphy SV, Atala A, 2014, 3D Bioprinting of Tissues and
rendered the thermogels with good extrudability while
maintaining the predesigned structures. Furthermore, Organs. Nat Biotechnol, 32:773–85.
the thermogels allowed for UV photopolymerization https://doi.org/10.1038/nbt.2958
to stabilize the printed scaffolds, with storage modulus 8. Jin Y, Liu C, Chai W, et al., 2017, Self-Supporting Nanoclay
dramatically increased. Through a two-step crosslinking as Internal Scaffold Material for Direct Printing of Soft
strategy, complicated constructs with high shape fidelity Hydrogel Composite Structures in Air. ACS Appl Mater
can be printed. Moreover, the self-supporting printed Interfaces, 9:17456–65.
scaffolds could be picked up by hand. The synthesis https://doi.org/10.1021/acsami.7b03613
process of the dual-sensitive hydrogels was simple and
low-cost, meaning that large-scale production in industrial 9. Lee A, Hudson AR, Shiwarski DJ, et al., 2019, 3D Bioprinting
manufacture was possible. The dual-sensitive hydrogels of Collagen to Rebuild Components of the Human Heart.
were expected to be promising bioinks, development of Science, 365:482–7.
cell-laden inks and their applications in tissue engineering 10. Yin J, Yan M, Wang Y, et al., 2018, 3D Bioprinting of Low-
will be explored in future studies. Concentration Cell-Laden Gelatin Methacrylate (GelMA)

Acknowledgments Bioinks with a Two-Step Cross-linking Strategy. ACS Appl
Mater Interfaces, 10:6849–57.
This work was financially supported by the National https://doi.org/10.1021/acsami.7b16059.s001
Key Research and Development Program of China 11. Colosi C, Shin SR, Manoharan V, et al., 2016, Microfluidic
(2017YFC1103400).
Bioprinting of Heterogeneous 3D Tissue Constructs Using
Conflict of interest Low-Viscosity Bioink. Adv Mater, 28:677–84.

All authors declare that they have no conflicts of interest. https://doi.org/10.1002/adma.201503310
12. Singh YP, Bandyopadhyay A, Mandal BB, 2019, 3D
Author contributions Bioprinting Using Cross-Linker-Free Silk-Gelatin Bioink for

Y. Z. designed the experiments. Y. Z. and Y. C. performed Cartilage Tissue Engineering. ACS Appl Mater Interfaces,
the experiments and analyzed the results. Y. Z. wrote the 11:33684–96.
manuscript. L. W. supervised the work and revised the https://doi.org/10.1021/acsami.9b11644
manuscript. 13. Schacht K, Jungst T, Schweinlin M, et al., 2015, Biofabrication

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