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3D-Printed β-TCP scaffolds promote Osteogenic Differentiation of BMSCs in an m6A-Dependent Manner
A
B
C D
Figure 4. m6A level of RUNX2 mRNA and the stability of RUNX2 mRNA after TCP treatment. (A) Possible sites with m6A modification
of RUNX2 mRNA. (B) Sequences of possible sites with m6A modification. (C) M6A level of RUNX2 mRNA in BMSCs in the Ctrl and
TCP groups. (D) The decay of RUNX2 mRNA after TCP treatment at 0, 2, 4, and 6 h. * P < 0.05; **P < 0.01.
Moreover, the expression levels of m6A-related enzymes not significantly different between the two groups,
were detected by immunohistochemistry. Identical to suggesting that METTL3 played a key role in the process
the in vitro results, the expression level of METTL3 on of osteogenesis induced by β-TCP (Figure 6E, G, H).
the TCP group was approximately 1.5-fold higher than All the results indicated that β-TCP increased osteogenic
that of the Ctrl group (Figure 6E and F). However, proteins in calvarial defect models, and METTL3 other
the expression levels of WTAP and ALKBH5 were than WTAP or ALKBH5 promoted the osteogenesis.
38 International Journal of Bioprinting (2022)–Volume 8, Issue 2
