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3D-Printed β-TCP scaffolds promote Osteogenic Differentiation of BMSCs in an m6A-Dependent Manner
of β-TCP in vivo, which shed light on the mechanism https://doi.org/10.1186/s12967-019-02131-y
of osteoinductivity of β-TCP from the perspective of 4. Chu W, Gan Y, Zhuang Y, et al., 2018, Mesenchymal Stem
epigenetic modifications. Cells and Porous β-tricalcium Phosphate Composites
5. Conclusions Prepared through Stem Cell Screen-enrich-combine
(Biomaterials) Circulating System for the Repair of Critical
In this study, we investigated the effect of β-TCP on Size Bone Defects in Goat Tibia. Stem Cell Res Ther, 9:157.
osteogenic differentiation of BMSCs. The underlying https://doi.org/10.1186/s13287-018-0906-1
mechanism is that β-TCP increases the expression of
METTL3, leading to a higher m6A level of RUNX2. The 5. Chu W, Wang X, Gan Y, et al., 2019, Screen-enrich-combine
rise of m6A level results in the retardation of decay and Circulating System to Prepare MSC/β-TCP for Bone Repair
enhanced stability of RUNX2 mRNA, causing an increase in Fractures with Depressed Tibial Plateau. Regener Med,
of RUNX2 mRNA and protein levels. As a result, RUNX2 14:555–69.
triggers the transcription of other osteogenic factors and https://doi.org/10.2217/rme-2018-0047
promotes osteogenic differentiation of BMSCs.
6. Wang X, Chu W, Zhuang Y, et al., 2019, Bone Mesenchymal
Funding Stem Cell-Enriched β-Tricalcium Phosphate Scaffold
Processed by the Screen-Enrich-Combine Circulating System
This work was supported by the National Key Research Promotes Regeneration of Diaphyseal Bone Non-Union. Cell
and Development Program of China (2017YFC110390),
National Natural Science Foundation of China Transplant, 28:212–23.
(82172402), Funds of the Clinical Research Plan of https://doi.org/10.1177/0963689718818096
SHDC (16CR3099B), and National Natural Science 7. Masaoka T, Yoshii T, Yuasa M, et al., 2016, Bone Defect
Foundation of China (82072412). Regeneration by a Combination of a β-Tricalcium Phosphate

Conflicts of interest Scaffold and Bone Marrow Stromal Cells in a Non-Human
Primate Model. Open Biomed Eng J, 10: 2-11.
All the authors declare no conflicts of interest. https://doi.org/10.2174/1874120701610010002

Authors’ contributions 8. Barradas AM, Monticone V, Hulsman M, et al., 2013, Molecular
Mechanisms of Biomaterial-driven Osteogenic Differentiation
Y.G. and J.W. designed the study. X.J. and X.S. performed in Human Mesenchymal Stromal Cells. Integr Biol, 5:920–31.
the experiments. X.J. and W.L. interpreted data and wrote
the manuscript. W.C., Y.Z., and Y.L. helped analyze https://doi.org/10.1039/c3ib40027a
the data. Z.W., X.Z., J.M., C.X., and K.D. provided 9. Liu J, Zhao L, Ni L, et al., 2015, The Effect of Synthetic
experimental assistance. Y.G. supervised the project. All α-tricalcium Phosphate on Osteogenic Differentiation of Rat
authors read and approved the manuscript. Bone Mesenchymal Stem Cells. Am J Transl Res, 7:1588–601.
10. Rittipakorn P, Thuaksuban N, Mai-Ngam K, et al., 2021,
References
Bioactivity of a Novel Polycaprolactone-Hydroxyapatite
1. Wang Z, Guo Z, Bai H, et al., 2013, Clinical Evaluation Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro
of β-TCP in the Treatment of Lacunar Bone Defects: Study. Polymers (Basel), 13:466.
A Prospective, Randomized Controlled Study. Mater Sci Eng https://doi.org/10.3390/polym13030466
C, 33:1894–9. 11. Hesse E, Saito H, Kiviranta R, et al., 2010, Zfp521 Controls
https://doi.org/10.1016/j.msec.2012.12.041 Bone Mass by HDAC3-dependent Attenuation of Runx2
2. Seebach C, Henrich D, Kähling C, et al., 2010, Endothelial Activity. J Cell Biol, 191:1271–83.
Progenitor Cells and Mesenchymal Stem Cells Seeded onto https://doi.org/10.1083/jcb.201009107
β-TCP Granules Enhance Early Vascularization and Bone 12. Liu JC, Lengner CJ, Gaur T, et al., 2011, Runx2 Protein
Healing in a Critical-Sized Bone Defect in Rats. Tissue Eng Expression Utilizes the Runx2 P1 Promoter to Establish
Part A, 16:1961–70. Osteoprogenitor Cell Number for Normal Bone Formation.
https://doi.org/10.1089/ten.tea.2009.0715 J Biol Chem, 286:30057–70.
3. Chu W, Zhuang Y, Gan Y, et al., 2019, Comparison and https://doi.org/10.1074/jbc.M111.241505
Characterization of Enriched Mesenchymal Stem Cells 13. Kronenberg HM, 2004, Twist Genes Regulate Runx2 and
Obtained by the Repeated Filtration of Autologous Bone Bone Formation. Dev Cell, 6:317–8.
Marrow Through Porous Biomaterials. J Transl Med, 17:377. https://doi.org/10.1016/S1534-5807(04)00069-3

42 International Journal of Bioprinting (2022)–Volume 8, Issue 2