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Liu, et al.
A B

C D

E

Figure 7. Functionality comparison of monolayer ARPE-19 models. Average permeability coefficients of (A) 40 kDa and (B) 500 kDa
FITC-dextran probes of S20, S50, and PET membrane without cell seeding and after 7 days of RPE seeding and culturing (n = 3). (C) TEER
and (D) TEP change over 3 weeks (n = 3). (E) Western blot results revealed the significant upregulation of Na /K -ATPase in EHDJ-printed
+
+
scaffold-based RPE models (n = 3). *P < 0.05, **P < 0.001, ***P < 0.0001, ****P < 0.00001.
FITC-dextran, the permeability coefficient of the S20 eyes has the much lower P for 40 kDa FITC-dextran
app
dropped to 12.74 × 10 cm s ~ 16.88 × 10 cm s. For S50, at around 0.46 × 10 cm s . Our results suggested
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P app was around 13.48 × 10 cm s after 7 days of culture. that permeability can be adjusted by changing the
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As a reference, the RPE-choroid complex in bovine preset pore size of EHDJ-printed scaffolds. Since RPE-
11 International Journal of Bioprinting (2022)–Volume 8, Issue 3