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Multifunctional 3D Bioprinting System for Construction of Complex Tissue Structure Scaffolds
A B C D

E

F

G H I

J K L M N

Figure 6. Initial experiments of printing thermosensitive hydrogels. The rheological properties of gelatin (A), GelMA (B), silica gel (C), and
PF127 (D). (E) A series of structures were printed using gelatin ink to verify the printability of the system. (F) PF127 (40% [w/t]) (Pluronic
F-127, Dow Corning) was printed at 18°C to verify the printer’s ability to print complex structures. Structures of grids (a, b, c), rings (d),
dolphins (e), dual-material stacked grids (f), alternately printed grids with dual nozzles (g), and three stacked grids printed with three nozzles
(h). (G) Rheological properties of gelatin (10% [w/t]) sodium alginate (1% [w/t]) bioinks. (H) Grid structures printed using gelatin (10%
[w/t]) sodium alginate (1% [w/t]). (I) Survival rate of printed cells (A549 [J], HeLa [K], NIH3T3 [L] and HUVECs [M]). (N) Actin staining
results of HeLa cells (day 7). Scale bar: 5 mm (E, F, H [a]), 100 μm (H [b]), 500 μm (J, K, L, M), and 200 μm (N).
3.2. Printing photosensitive hydrogel will be used as a photosensitive hydrogel bioink to
GelMA is a commonly used photosensitive biomaterial, verify the printing ability of the cell 3D printing system
which is gelatin methacrylated. In this section, GelMA developed in this paper in terms of photosensitive ink.

264 International Journal of Bioprinting (2022)–Volume 8, Issue 4