Page 44 - IJB-9-1

International Journal of Bioprinting

RESEARCH ARTICLE
Effect of ionic crosslinking on morphology and

thermostability of biomimetic supercritical
fluids-decellularized dermal-based composite

bioscaffolds for bioprinting applications

5,6
Run-Miao Yang 1,2† , Jun Xu 2,3,4† , Ching-Cheng Huang *
1 Department of Material Engineering, Jiangsu University of Technology, Chang Zhou, Jiangsu, China
2 PARSD Biomedical Material Research Center (Chang Zhou), Chang Zhou, Jiangsu, China
3 College of Food Science and Engineering, Hainan University; Engineering Research Center
of Utilization of Tropical Polysaccharide Resources, Ministry of Education, Haikou 570 228,
Hainan, China
4 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, CAS 100 190,
Beijing, China
5 Department of Biomedical Engineering, Ming-Chuan University, Taoyuan 320-33, Taiwan
6 PARSD Biomedical Material Research Center (Taiwan), Taichung, Taiwan

Abstract
† These authors contributed equally
to this work. In the present study, supercritical fluid was employed to prepare a kind of
*Corresponding author: supercritical fluids-decellularized dermal-based scaffold (SFDDS) from porcine
Ching-Cheng Huang dermal tissue. Further, new composite bioscaffolds containing SFDDS were designed
(junas.tw@yahoo.com.tw)
for bioprinting applications. Then, the effect of crosslinking functionality on
Citation: Yang R, Xu J, Huang C, microstructures and thermal properties of the composite bioscaffolds containing
2023, Effect of Ionic crosslinking
on morphology and thermostability decellularized extracellular matrix were studied. The results of thermal stability
of biomimetic supercritical from thermogravimetric analysis and difference thermogravimetry demonstrated
fluids-decellularized dermal- the structural stability of the composite bioscaffolds. A method was designed to
based composite bioscaffolds
for bioprinting applications. Int J prepare bioinspired decellularized dermal-based composite bioscaffolds, which
Bioprint, 9(1): 625. were further characterized by infrared spectroscopy, scanning electron microscopy,
http://doi.org/10.18063/ijb.v9i1.625 and thermogravimetry analysis.
Received: April 27, 2022;
Accepted: August 11, 2022; Keywords: Alginate; Composite membrane; Decellularization; Microstructure;
Supercritical carbon dioxide
Published Online: October 27,
2022
Copyright: © 2022 Author(s).
This is an Open Access article 1. Introduction
distributed under the terms of the
Creative Commons Attribution Numerous natural or modified materials with specific microstructures have been
License, permitting distribution,
and reproduction in any medium, studied for medical or bioprinting applications, for instance, the application of bioinks
provided the original work is or bioscaffolds for tissue reconstruction [1-8] . A wide range of materials with varied
properly cited. viscosities and high cell density aggregates can be 3D printed using this technique .
[2]
Publisher’s Note: Whioce A large variety of polymers is under research for the use in bioprinting technology.
Publishing remains neutral with Natural polymers, including collagen , gelatin , alginate , and hyaluronic acid ,
[3]
[4]
[6]
[5]
regard to jurisdictional claims in [7]
published maps and institutional and synthetic polymers, such as polyvinyl alcohol (PVA) and polyethylene glycol,
affiliations. are commonly used in bioinks for 3D printing. Often, these bioinks are post-processed
Volume 9 Issue 1 (2023) 36 http://doi.org/10.18063/ijb.v9i1.625