Page 300 - IJB-9-2

International Journal of Bioprinting

RESEARCH ARTICLE
Zn-doped chitosan/alginate multilayer

coatings on porous hydroxyapatite scaffold
with osteogenic and antibacterial properties

1,2
3

1,2
Zhijing He 1,2† , ChenJiao 1,2† , Junnan Wu , Jiasen Gu , Huixin Liang ,
5
Lida Shen 1,2,4 *, Youwen Yang *, Zongjun Tian 1,2,4 , Changjiang Wang ,
4
Qing Jiang 6
1 Institute of Additive Manufacturing (3D Printing), Nanjing University of Aeronautics and Astronautics,
Nanjing, 210016, China
2 College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and
Astronautics, Nanjing, 210016, China
3 State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University Medical School Affiliated
Nanjing Drum Tower Hospital, Nanjing, 210008, China
4 Institute of Additive Manufacturing, Jiangxi University of Science and Technology, Ganzhou,
341000, China
5 Department of Engineering and Design, University of Sussex, Brighton, BN1 9RH, United Kingdom
6 State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult
Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The
Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, China
† These authors contributed equally
to this work.
*Corresponding authors:
Lida Shen
(ldshen@nuaa.edu.cn) Abstract
Youwen Yang
(yangyouwen@jxust.edu.cn) Porous hydroxyapatite (HA) scaffolds prepared by three-dimensional (3D) printing
Citation: He Z, Jiao C, Wu J, have wide application prospects owing to personalized structural design and
et al., 2023, Zn-doped chitosan/ excellent biocompatibility. However, the lack of antimicrobial properties limits its
alginate multilayer coatings on widespread use. In this study, a porous ceramic scaffold was fabricated by digital
porous hydroxyapatite scaffold
with osteogenic and antibacterial light processing (DLP) method. The multilayer chitosan/alginate composite
2+
properties. Int J Bioprint, 9(2): 668. coatings prepared by layer-by-layer method were applied to scaffolds and Zn
https://doi.org/10.18063/ijb.v9i2.668 was doped into coatings in the form of ion crosslinking. The chemical composition
Received: September 7, 2022 and morphology of coatings were characterized by scanning electron microscope
(SEM) and X-ray photoelectron spectroscopy (XPS). Energy dispersive spectroscopy
Accepted: October 21, 2022
(EDS) analysis demonstrated that Zn was uniformly distributed in the coating.
2+
Published Online: January 13, Besides, the compressive strength of coated scaffolds (11.52 ± 0.3 MPa) was slightly
2023
improved compared with that of bare scaffolds (10.42 ± 0.56 MPa). The result of
Copyright: © 2023 Author(s). soaking experiment indicated that coated scaffolds exhibited delayed degradation.
This is an Open Access article In vitro experiments demonstrated that within the limits of concentration,
distributed under the terms of the
Creative Commons Attribution a higher Zn content in the coating has a stronger capacity to promote cell
License, permitting distribution, adhesion, proliferation and differentiation. Although excessive release of Zn led
2+
and reproduction in any medium, to cytotoxicity, it presented a stronger antibacterial effect against Escherichia coli
provided the original work is
properly cited. (99.4%) and Staphylococcus aureus (93%).
Publisher’s Note: Whioce
Publishing remains neutral with Keywords: Porous hydroxyapatite scaffold; Multi-layer polymer coating; Zn doping;
regard to jurisdictional claims in
published maps and institutional Osteogenic property; Antibacterial property
affiliations.

Volume 9 Issue 2 (2023) 292 https://doi.org/10.18063/ijb.v9i2.668