Page 339 - IJB-9-2

International Journal of Bioprinting

RESEARCH ARTICLE
Preparation and characterization of

3D-printed antibacterial hydrogel with benzyl
isothiocyanate

1†
Yunxia Liang , Bimal Chitrakar , Zhenbin Liu , Xujia Ming , Dan Xu ,
1
1
2†
1
1
1
1
1
Haizhen Mo , Chunyang Shi , Xiaolin Zhu , Liangbin Hu *, Hongbo Li *
1
1 School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an,
710021, China
2 College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, Hebei, China
(This article belongs to the Special Issue: Related to 3D printing technology and materials)
Abstract
Benzyl isothiocyanate (BITC) is an isothiocyanate of plant origin, especially the
mustard family, which has good antibacterial properties. However, its applications
are challenging due to its poor water solubility and chemical instability. We
used food hydrocolloids, including xanthan gum, locust bean gum, konjac
glucomannan, and carrageenan as three-dimensional (3D)-printing food ink base
† These authors contributed equally and successfully prepared 3D-printed BITC antibacterial hydrogel (BITC-XLKC-Gel).
to this work. The characterization and fabrication procedure of BITC-XLKC-Gel was studied. The
*Corresponding authors: results show that BITC-XLKC-Gel hydrogel has better mechanical properties by low-
Liangbin Hu field nuclear magnetic resonance (LF-NMR), mechanical properties, and rheometer
(hulb@sust.edu.cn)
Hongbo Li analysis. The strain rate of BITC-XLKC-Gel hydrogel is 76.5%, which is better than
(hongbo715@163.com) that of human skin. Scanning electron microscope (SEM) analysis showed that
BITC-XLKC-Gel has uniform pore size and provides a good carrier environment for
Citation: Liang Y, Chitrakar B,
Liu Z, 2023, Preparation and BITC carriers. In addition, BITC-XLKC-Gel has good 3D-printing performance, and
characterization of 3D-printed 3D printing can be used for customizing patterns. Finally, inhibition zone analysis
antibacterial hydrogel with showed that the BITC-XLKC-Gel added with 0.6% BITC had strong antibacterial
benzyl isothiocyanate. Int J
Bioprint, 9(2): 671. activity against Staphylococcus aureus and the BITC-XLKC-Gel added with 0.4% BITC
https://doi.org/10.18063/ijb.v9i2.671 had strong antibacterial activity against Escherichia coli. Antibacterial wound dressing
Received: September 9, 2022 has always been considered essential in burn wound healing. In experiments that
simulated burn infection, BITC-XLKC-Gel showed good antimicrobial activity against
Accepted: November 8, 2022
methicillin-resistant S. aureus. BITC-XLKC-Gel is a good 3D-printing food ink attributed
Published Online: January 17, 2023 to strong plasticity, high safety profile, and good antibacterial performance and has
Copyright: © 2023 Author(s). great application prospects.
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution Keywords: Antibacterial hydrogel; Benzyl isothiocyanate; 3D printing; Rheological
License, permitting distribution, properties; Staphylococcus aureus; Escherichia coli
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: Whioce
Publishing remains neutral with 1. Introduction
regard to jurisdictional claims in
published maps and institutional Benzyl isothiocyanate (BITC) is an isothiocyanate of plant origin, which has good
[1]
affiliations. antibacterial properties . BITC is potentially beneficial to human health. It is widely found

Volume 9 Issue 2 (2023) 331 https://doi.org/10.18063/ijb.v9i2.671