Page 388 - IJB-9-2

International Journal of Bioprinting

RESEARCH ARTICLE
Commercial articulated collaborative in situ 3D

bioprinter for skin wound healing

1
1†
Aleksandr A. Levin *, Pavel A. Karalkin 2,3† *, Elizaveta V. Koudan ,
4
1
Fedor S. Senatov , Vladislav A. Parfenov , Vladislav A. Lvov ,
1
1
Stanislav V. Petrov , Frederico D. A. S. Pereira , Alexey V. Kovalev ,
6
5
Egor O. Osidak , Sergey P. Domogatsky , Natalya E. Manturova ,
7
9
7,8
Vladimir A. Kasyanov , Natalia S. Sergeeva , Vadim L. Zorin 11,12 ,
10
2
Yusef D. Khesuani , Vladimir A. Mironov 1,6
5
1 Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Moscow,
Russia
2 National Medical Research Radiological Center, P. A. Hertsen Moscow Oncology Research Center,
Moscow, Russia
3 L. L. Levshin Institute of Cluster Oncology, Sechenov First Moscow State Medical University,
Moscow, Russia
4 A. A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Moscow,
Russia
5 Laboratory for Biotechnological Research “3D Bioprinting Solutions”, Moscow, Russia
6 Priorov Central National Institute of Traumatology and Orthopedics, Moscow, Russia
7 Imtek Ltd., Moscow, Russia
8 Dmitry Rogachev National Medical Research Center of Paediatric Haematology, Oncology and
† These authors contributed equally Immunology, Moscow, Russia
to this work. 9 JSC Plastic Surgery and Cosmetology Institute, Moscow, Russia
*Corresponding authors: 10 Joint Laboratory of Traumatology and Orthopedics, Riga Stradins University, Riga, Latvia
Aleksandr A. Levin
(levin.alexandr.stankin@gmail.com) 11 Human Stem Cells Institute, Moscow, Russia
Pavel A. Karalkin 12 SKINCELL LLC, Skolkovo, Moscow, Russia
(pkaralkin@gmail.com)
Citation: Levin AA, Karalkin PA,
Koudan EV, 2023, Commercial
articulated collaborative in situ 3D Abstract
bioprinter for skin wound healing.
Int J Bioprint. 9(2): 675.
https://doi.org/10.18063/ijb.v9i2.675 In situ bioprinting is one of the most clinically relevant techniques in the emerging
bioprinting technology because it could be performed directly on the human body
Received: December 1, 2022
in the operating room and it does not require bioreactors for post-printing tissue
Accepted: December 13, 2022 maturation. However, commercial in situ bioprinters are still not available on the
Published Online: January 31, 2023 market. In this study, we demonstrated the benefit of the originally developed
first commercial articulated collaborative in situ bioprinter for the treatment of
Copyright: © 2023 Author(s).
This is an Open Access article full-thickness wounds in rat and porcine models. We used an articulated and
distributed under the terms of the collaborative robotic arm from company KUKA and developed original printhead
Creative Commons Attribution and correspondence software enabling in situ bioprinting on curve and moving
License, permitting distribution,
and reproduction in any medium, surfaces. The results of in vitro and in vivo experiments show that in situ bioprinting of
provided the original work is bioink induces a strong hydrogel adhesion and enables printing on curved surfaces
properly cited. of wet tissues with a high level of fidelity. The in situ bioprinter was convenient to use
Publisher’s Note: Whioce in the operating room. Additional in vitro experiments (in vitro collagen contraction
Publishing remains neutral with assay and in vitro 3D angiogenesis assay) and histological analyses demonstrated
regard to jurisdictional claims in
published maps and institutional that in situ bioprinting improves the quality of wound healing in rat and porcine skin
affiliations. wounds. The absence of interference with the normal process of wound healing and

Volume 9 Issue 2 (2023) 380 https://doi.org/10.18063/ijb.v9i2.675