Page 221 - IJB-9-3

International Journal of Bioprinting Dual ions mixed GelMA for hair follicle regeneration

only has a stimulatory effect on stem cells, but also can Ethics approval and consent to participate
promote angiogenesis and perfusion recovery, indicating Animals were maintained in a specific-pathogen-free
that there are direct and indirect manners to regulate hair facility of Chinese PLA General Hospital in accordance
follicle regeneration. Although further work is required to with the Guide for the Care and Use of Laboratory Animals.
illuminate the molecular mechanisms, integrating Zn/Si All animal experiments were approved by the Institutional
dual ions with GelMA hydrogel is an attractive option that Animal Care and Use Committee of Chinese PLA General
opens new avenues for hair follicle regenerative skin repair.
Hospital (approval number SCXK(BJ)2017–0019).
5. Conclusion Consent for publication

In summary, we describe a feasible method to combine
GelMA with Zn/Si dual ions to promote in situ hair Not applicable.
follicle regeneration with the assistance of 3D bioprinting
technology. Collectively, our in vitro and in vivo data Availability of data
underscore the capabilities of Zn/Si ions to impact the The data that support the findings of this study are available
behavior of HFSCs and endothelial cells during hair from the corresponding authors upon reasonable request.
follicle regeneration, which may directly or indirectly
contribute to microenvironment manipulation of cell References
fate. The research also offers insights into promoting hair 1. Gurtner GC, Werner S, Barrandon Y, et al., 2008, Wound
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characterization. https://doi.org/10.1101/cshperspect.a023267
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Funding Physiological regeneration of skin appendages and
This study was supported by the Science Fund for National implications for regenerative medicine. Physiology,
27(2):61–72.
Defense Distinguished Young Scholars (2022-JCJQ-
ZQ-016), Key basic research projects of the Foundation https://doi.org/10.1152/physiol.00028.2011
Strengthening Plan (2022-JCJQ-ZD-096-00), National 4. Ito M, Yang Z, Andl T, et al., 2007, Wnt-dependent de
Key Research and Development Program of China novo hair follicle regeneration in adult mouse skin after
(2022YFA1104604), National Natural Science Foundation wounding. Nature, 447(7142):316–320.
of China (32000969), and Key Support Program for https://doi.org/10.1038/nature05766
Growth Factor Research (SZYZ-TR-03).
5. Lim CH, Sun Q, Ratti K, et al., 2018, Hedgehog stimulates
Conflict of interest hair follicle neogenesis by creating inductive dermis during
murine skin wound healing. Nat Commun, 9(1):4903.
The authors declare no conflict of interest. https://doi.org/10.1038/s41467-018-07142-9

Author contributions 6. Xing M, Jiang Y, Bi W, et al., 2021, Strontium ions protect
hearts against myocardial ischemia/reperfusion injury. Sci
Conceptualization: Fanliang Zhang, Sha Huang, Bin Yao, Adv, 7(3):eabe0726.
Jiang Chang https://doi.org/10.1126/sciadv.abe0726
Formal analysis: Fanliang Zhang, Xianlan Duan, Zhao Li 7. Huang T, Zhang T, Jiang X, et al., 2021, Iron oxide
Funding acquisition: Sha Huang, Xiaobing Fu nanoparticles augment the intercellular mitochondrial
Investigation: Fanliang Zhang, Zhaowenbin Zhang, Xianlan transfer-mediated therapy. Sci Adv, 7(40):eabj0534.
Duan, Yi Kong, Xing Huang
Methodology: Zhao Li, Wei Song, Bin Yao, Sha Huang, Yi https://doi.org/10.1126/sciadv.abj0534
Kong 8. Zhang Z, Li W, Liu Y, et al., 2021, Design of a biofluid-
Resources: Zhaowenbin Zhang, Wei Song, Xing Huang, absorbing bioactive sandwich-structured Zn-Si bioceramic
Jiang Chang composite wound dressing for hair follicle regeneration and
Writing – original draft: Fanliang Zhang, Sha Huang skin burn wound healing. Bioact Mater, 6(7):1910–1920.
Writing – review & editing: Sha Huang, Xiaobing Fu https://doi.org/10.1016/j.bioactmat.2020.12.006

Volume 9 Issue 3 (2023) 213 https://doi.org/10.18063/ijb.703