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International Journal of Bioprinting Dual ions mixed GelMA for hair follicle regeneration

Figure 5. GelMA-Zn/Si hydrogel enhances in situ regeneration of hair follicle in mice excisional models. Gross appearance of the skin wounds. (A) Images
were captured at 0, 7, and 14 days after operation. (B) Quantification of wound area. **P < 0.01. The diameter of the rubber ring is 1 cm.

the wound tissue. The results showed that on the 14th 3.7. Gene expression
day, GelMA-Zn/Si group had more positive staining in To further investigate the effect of GelMA-Zn/Si hydrogel
the wound areas, while less yellow staining was found in on hair follicle regeneration and angiogenesis, on the 14th
the other groups (Figure 6D). The quantitative analyses day, the wound tissue was harvested and used for qRT-
confirmed that GelMA-Zn/Si hydrogel group had PCR (Figure 7). We determined the expression of hair
the highest neovascularization, whereas GelMA and follicle anagen markers, such as Pdgfa and Pdgfb . The
[31]
hydrocolloid groups only had a small amount of neogenic results indicated that GelMA-Zn/Si hydrogel group had
vessels (Figure 6E). These findings indicated that GelMA- the highest expression level, which means the hydrogel
Zn/Si hydrogel has stimulatory effects on angiogenesis. may stimulate hair follicle regeneration. The stem cell

Volume 9 Issue 3 (2023) 208 https://doi.org/10.18063/ijb.703