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International Journal of Bioprinting Dual ions mixed GelMA for hair follicle regeneration

Figure 6. (A) Representative H&E-stained sections on the 14th day after treatment. Black arrows point to the neogenic hair follicles. (B) Representative
immunofluorescence images on the 14th day after treatment show the presence of K19 cells. (C) Quantitative analysis of the number of K19 cells.
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(D) Representative immunofluorescence images on the 14th day after treatment show the presence of CD31/α-SMA vessels. (E) Quantitative analysis of
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CD31/α-SMA intensity (K19, green; CD31, red; α-SMA, green; DAPI, blue). N = 3 for each group. All the analyses were performed with one-way ANOVA.
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Scale bar = 200 μm (A); 100 μm (B, D).
markers, such as Kgf and Egf, were also detected in the is related to angiogenesis. Insulin-like growth factor 1 is
study, and GelMA-Zn/Si hydrogel group had a stronger an important growth factor that stimulates endothelial
expression than others, suggesting that GelMA-Zn/Si cell migration and regulates neovascularization . Similar
[33]
hydrogel may promote the regeneration activity of the to the results of Vegf, GelMA-Zn/Si hydrogel strongly
tissue . Compared with other groups, GelMA-Zn/Si upregulated the expression of Igf1 and the data implies that
[32]
hydrogel greatly upregulated the expression of Vegf, which GelMA-Zn/Si hydrogel may accelerate the formation of

Volume 9 Issue 3 (2023) 209 https://doi.org/10.18063/ijb.703