Page 401 - IJB-9-3

International Journal of Bioprinting Bioprinting of a multicellular model

A D

B E

C F

Figure 12. Dose response curve of 5-FU (A), oxaliplatin (B), and irinotecan (C) between 2D culture group and 3D Printing-S group after 72 h of treatment.
IC represents median inhibitory concentration. Dose-effect curves of 5-FU (D), oxaliplatin (E), and irinotecan (F) in the 3D printing-M after 72 h of
50
treatment.

well in the 3D printing-M and that the constructed 3D 3D printing-S group. GO enrichment analysis indicated
printing-M of mesenchymal tumor cells was successful. that compared with colorectal cancer cells cultured in
In addition, through exploration and attempts, we were 2D culture, the gene expression of colorectal cancer
able to produce frozen tissue sections of 3D bioprinted cells cultured in a 3D bioprinted model was significantly
models and understand the morphological characteristics different in biological processes, cell composition, and
[39]
of tumor cells and interstitial cells through HE staining of molecular function . KEGG enrichment analysis showed
the frozen sections. The results show that the cell lines have that compared with the 2D culture group, metabolic
a certain degree of tissue characteristics depending on the pathway, and oxidative phosphorylation pathway were the
bionic characteristics of the 3D bioprinting technology. most significantly upregulated gene enrichment pathways
in the 3D printing-S group [40-42] . These results suggest
From the perspective of transcriptome, we analyzed the that colorectal cancer cells in the 3D bioprinted model
differences in gene expression among the 3D printing-S are significantly different from those in the 2D cultures.
group, 2D culture group, sandwich culture group, and 3D The main sources of these differences are the different
printing-M group. As can be seen from the volcano map of cell culture models, the different structures of the cell
DEGs, the number of DEGs in SW480 cells was as high as survival space, and the different degrees of communication
11107 between the two culture models of 2D culture and between cells. The functions of the hub genes between the

Volume 9 Issue 3 (2023) 393 https://doi.org/10.18063/ijb.694