Page 88 - IJB-9-3

International Journal of Bioprinting

RESEARCH ARTICLE
Fabrication and characterization of

photosensitive non-isocyanate polyurethane
acrylate resin for 3D printing of customized

biocompatible orthopedic surgical guides

Yan Wang 1,2,3† , Zhichao Zheng , Janak L. Pathak , Weiwei Feng 1,2,3 ,
4†
4†
Weicong Wu 1,2,3 , Chuangang Yang 1,2,3 , Lihong Wu *, Huade Zheng 1,2,3,5 *
4
1 School of Materials Science and Engineering, South China University of Technology, Guangzhou
510006, China
2 National Engineering Research Center for Tissue Restoration and Reconstruction, South China
University of Technology, Guangzhou 510006, China
3
Key Laboratory of Biomedical Engineering of Guangdong Province and Innovation Center for
Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006,
China
4 Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering
Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and
Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong 510182, China
5 South China Institute of Collaborative Innovation, Dongguan 523808, China
† These authors contributed equally
to this work.
*Corresponding authors:
Huade Zheng Abstract
(hdzheng@scut.edu.cn)
Lihong Wu Three-dimensional (3D)-printed orthopedic surgical guides have the potential to
(wcanhong@163.com)
provide personalized precision treatment. Non-isocyanate polyurethane (NIPU)
Citation: Wang Y, Zheng Z, is commonly used in the 3D printing of biomedical materials but its application
Pathak JL, et al., 2023, in the orthopedic surgical guide is limited by poor mechanical properties and
Fabrication and characterization
of photosensitive non-isocyanate biocompatibility. In this study, we fabricated non-isocyanate polyurethane acrylate
polyurethane acrylate resin for 3D (NIPUA) photosensitive resin with superior biocompatibility and mechanical
printing of customized biocompatible properties required for 3D-printed orthopedic surgical guides. NIPU prepolymer
orthopedic surgical guides.
Int J Bioprint, 9(3): 684. was synthesized by a ring-opening reaction and a ring acrylation reaction. NIPUA
https://doi.org/10.18063/ijb.684 was further synthesized using polyethylene glycol diacrylate (PEGDA) as a modified
Received: October 08, 2022 material based on sustainable synthesis with reduced synthesis time. NIPUA showed
Accepted: November 08, 2022 the best tensile and flexural strengths when the PEGDA content reached 12 wt.%. NIPUA
Published Online: February 14, exhibited higher thermal stability, hemocompatibility, superior biocompatibility
2023
to ME3T3-E1 bone cells and C1C12 muscle cells, and non-immunogenic effect
Copyright: © 2023 Author(s). toward macrophages compared with commercial photosensitive resins. Commercial
This is an Open Access article
distributed under the terms of the resins triggered a severe inflammatory response during in vivo implantation, but
Creative Commons Attribution this effect was not observed during NIPUA implantation. Transcriptome analysis
License, permitting distribution showed downregulation of cell death and cell cycle disruption-related genes, such
and reproduction in any medium,
provided the original work is as CDK2, CDKN1a, and GADD45a, and upregulation of autophagy and anti-tumor
properly cited. activity-related genes, such as MYC, PLK1, and BUB1b, in NIPUA-treated MC3T3-E1
cells compared with commercial resin-treated MC3T3-E1 cells. In conclusion,
Publisher’s Note: Whioce
Publishing remains neutral with NIPUA resin showed excellent mechanical and thermal properties as well as good
regard to jurisdictional claims in biocompatibility toward bone cells, muscle cells, and macrophages, suggesting its
published maps and institutional possible application in the 3D printing of customized orthopedic surgical guides.
affiliations.

Volume 9 Issue 3 (2023) 80 https://doi.org/10.18063/ijb.684