Page 285 - IJB-9-5

International Journal of Bioprinting

REVIEW ARTICLE
Blood-derived biomaterials for tissue graft

biofabrication by solvent-based extrusion
bioprinting

Cristina Del Amo , Isabel Andia *
1
1,2
1 Regenerative Therapies, Biocruces Bizkaia Health Research Institute, Plaza Cruces s/n, 48903
Barakaldo, Spain
2 3D Printing and Bioprinting Laboratory, Biocruces Bizkaia Health Research Institute, Plaza Cruces
s/n, 48903 Barakaldo, Spain

Abstract

This article provides an overview of the different types of blood-derived biomaterials
that can be used as solvent additives in the formulation of inks/bioinks for use
in solvent extrusion printing/bioprinting. We discuss the properties of various
blood sub-products obtained after blood fractionation in terms of their use in
tailoring ink/bioink to produce functional constructs designed to improve tissue
repair. Blood-derived additives include platelets and/or their secretome, including
signaling proteins and microvesicles, which can drive cell migration, inflammation,
angiogenesis, and synthesis of extracellular matrix proteins. The contribution of
plasma to ink/bioink functionalization relies not only on growth factors, such as
hepatocyte growth factor and insulin growth factors, but also on adhesive proteins,
such as fibrinogen/fibrin, vitronectin, and fibronectin. We review the current
developments and progress in solvent-based extrusion printing/bioprinting with
*Corresponding author: inks/bioinks functionalized with different blood-derived products, leading toward
Isabel Andia
(isabel.andiaortiz@osakidetza.eus) the development of more advanced patient-specific 3D constructs in multiple
medical fields, including but not limited to oral tissues and cartilage, bone, skin,
Citation: Del Amo C, Andia I, 2023,
Blood-derived biomaterials for tissue liver, and neural tissues. This information will assist researchers in identifying the
graft biofabrication by solvent-based most suitable blood-derived product for their ink/bioink formulation based on the
extrusion bioprinting. Int J Bioprint, intended regenerative functionality of the target tissue.
9(5): 762.
https://doi.org/10.18063/ijb.762
Received: February 22, 2023 Keywords: Blood-derived products; 3D printing; Solvent-based extrusion;
Accepted: March 25, 2023 Bioprinting; Functionalized bioinks; Plasma; Tissue grafts/implants
Published Online: May 26, 2023
Copyright: © 2023 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution 1. Introduction
License, permitting distribution,
and reproduction in any medium, In the context of therapeutic tissue engineering (TE) and regenerative medicine
provided the original work is (RM), biofabrication entails the automated production of complex living and non-
properly cited. living biologically relevant products manufactured from living cells, native matrices,
Publisher’s Note: Whioce biomaterials, and molecules using different devices [1,2] . Here, we focus on one of the
Publishing remains neutral with most commonly used technologies, solvent-based extrusion (SBE) (bio)printing. In this
regard to jurisdictional claims in context, solvents containing printable biomaterial composites (chiefly hydrogels) and
published maps and institutional
affiliations. optional additives are extruded through a nozzle in a layer-by-layer additive manner using

Volume 9 Issue 5 (2023) 277 https://doi.org/10.18063/ijb.762