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REVIEW ARTICLE
Advancing organoid development with 3D

bioprinting

Wenping Ma 1,2 , Hongxu Lu * , Yin Xiao 3,4 , and Chengtie Wu *
1,2
1,2
1 State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of
Sciences, Shanghai, People’s Republic of China
2 Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, People’s Republic of
China
3 School of Medicine and Dentistry, Griffith University, Gold Coast, Australia
4 Institute for Biomedicine and Glycomics, Griffith University, Gold Coast, Australia
*Corresponding authors: Hongxu Lu (hongxu.lyu@mail.sic.ac.cn); Chengtie Wu (chengtiewu@mail.sic.ac.cn)

Citation: Ma W, Lu H, Xiao Y, Abstract
Wu C. Advancing organoid
development with 3D bioprinting.
Organoid Res. 2025;1(1):025040004. Organoids provide more realistic in vitro models that closely mimic their corresponding
doi: 10.36922/OR025040004 tissues or organs. Three-dimensional (3D) bioprinting allows for precise control over
cell distribution, arrangement, and the regulation of cell behavior and interactions,
Received: October 11, 2024
offering flexibility and repeatability. By combining 3D bioprinting with organoid
Accepted: December 15, 2024 culture, optimal extracellular matrix conditions can be created, better replicating
Published online: March 3, 2025 the complex interactions between cells and their environment. The integration of
3D bioprinting amplifies the scalability of organoids, facilitating the development
Copyright: © 2025 Author(s).
This is an Open-Access article of large-scale organ models that significantly enhance research capabilities. This
distributed under the terms of the review explores the crucial role of 3D bioprinting in organoid development,
Creative Commons Attribution emphasizing its contributions to improving organoid structure and functionality.
License, permitting distribution, and
reproduction in any medium, which We discuss how this innovative approach supports the creation of well-defined
provided that the original work is physiological microenvironments, aids in the development of functional organoids,
properly cited. and enables high-throughput cultivation. Furthermore, we highlight the advantages
Publisher’s Note: AccScience of optimizing bioprinting strategies and bioinks to advance organoid applications. In
Publishing remains neutral with regard addition, we examine the importance of refining 3D bioprinting devices to enhance
to jurisdictional claims in published
maps and institutional affiliations. organoid fabrication and application. Finally, we outline strategies for leveraging 3D
bioprinting to further advance organoid research and its implications for regenerative
medicine and disease modeling.

Keywords: 3D bioprinting; Organoid; Bioinks; Stem cells

1. Introduction small intestine. Since then, various organoids, including
3
liver, stomach, kidney, brain, and heart organoids, have
Over the past decade, organoids have gained significant been developed. Due to their similarity to real tissues and
4-9
attention in stem cell research and biomedical sciences. the aggregation and interaction of multiple organ-specific
Organoids are three-dimensional (3D) multicellular cells, organoids possess broad biomedical application
aggregates derived from stem cells or organ-specific prospects and have been studied for drug screening,
progenitor cells, which can differentiate and self- disease modeling, tissue development research, and tissue
organize to replicate specific structures and functions of regeneration. 10-12
corresponding organs. In 2009, Hans Clevers reported
1,2
the first small intestinal organoid derived from leucine-rich Given their importance as biomedical research tools,
repeat-containing G-protein coupled receptor 5-positive promoting the development and application of organoids
intestinal stem cells. This work revealed cell composition is essential. 3D bioprinting is a promising fabrication
and structural characteristics similar to those of the natural technology that has received increasing attention in

Volume 1 Issue 1 (2025) 1 doi: 10.36922/OR025040004