Page 51 - TD-2-1

Tumor Discovery

ORIGINAL RESEARCH ARTICLE
A novel gene prognostic signature lymphocyte

cytosolic protein 2 regulates melanoma
progression by activating tumor-infiltrating

CD8 T-cells through the interferon regulatory
+
factor 5 signaling pathway

6
4†
5
Hongyin Sun 1,2,3† , Kui Deng , Xingchen Zhou 1,2† , Dongsheng Cao , Yan Cheng ,
1,2
1,2
Xiang Chen *, and Mingzhu Yin *
1 Department of Dermatology, Hunan Engineering Research Center of Skin Health and Disease,
Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University,
Changsha, China
2 National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South
University, Changsha, China
3 Department of Pharmacy, Shantou University Medical College, Shantou University, Shantou, China
4 Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of
Life Sciences, Westlake University, 18 Shilongshan Rd, Cloud Town, Hangzhou, China
5 Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan, China
† These authors contributed equally 6 Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha,
to this work. China
*Corresponding authors:
Xiang Chen
(chenxiangck@126.com)
Mingzhu Yin Abstract
(yinmingzhu@csu.edu.cn)
Citation: Sun H, Deng K, Cutaneous malignant melanoma is the most lethal skin cancer. The advent of
Zhou X, et al., 2023, A novel gene immunotherapy has revolutionized the status of clinical therapies of melanoma, which
prognostic signature lymphocyte
cytosolic protein 2 regulates brought new hope to these patients. However, only a small proportion of patients
melanoma progression by activating are responders. Therefore, the identification of novel prognostic and immune-related
tumor-infiltrating CD8 T-cells biomarkers is crucial to guide the development of melanoma clinical treatments. Herein,
+
through the interferon regulatory
factor 5 signaling pathway. Tumor RNA-seq data of the cutaneous melanoma from public database were used for identifying
Discov, 2(1): 318. prognostic gene signatures, and we found that high expression of lymphocyte cytosolic
https://doi.org/10.36922/td.318
protein 2 (LCP2) in melanoma patient was significantly associated with better prognosis
Received: December 29, 2022 for melanoma. Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses
Accepted: February 27, 2023 demonstrated that the differentially expressed genes are significantly involved in
Published Online: March 24, 2023
lysosome, B-cell receptor signaling pathways, Fc epsilon RI signaling pathway, and T-cell
Copyright: © 2023 Author(s). receptor signaling pathway, indicating that these signaling pathways play important
This is an Open Access article
+
distributed under the terms of the roles in melanoma. LCP2 expression was positively correlated with CD8 T-cell and
Creative Commons Attribution the overall survival of melanoma patients, and this positive correlation was directly
License, permitting distribution, confirmed by fluorescence-activated cell sorting experiment. The in vivo experiment
and reproduction in any medium,
provided the original work is showed that LCP2 knockdown significantly promoted the melanoma progression
properly cited. and decreased interferon regulatory factor 5 (IRF5) expression. In conclusion, we
Publisher’s Note: AccScience identified that LCP2 is a possible prognostic gene signature for progression-free survival
Publishing remains neutral with of melanoma patients and regulates melanoma progression by activating tumor-
regard to jurisdictional claims in +
published maps and institutional infiltrating CD8 T-cells through the IRF5 signaling pathway, indicating that LCP2 could
affiliations. serve as a prognostic biomarker and therapeutic target in immunotherapy.

Volume 2 Issue 1 (2023) 1 https://doi.org/10.36922/td.318