Page 37 - TD-3-1

Tumor Discovery

ORIGINAL RESEARCH ARTICLE
Profiling energy metabolism in normal bladder

tissue and non-muscle-invasive bladder cancer
cases of different histological grades

Guilherme Prado Costa , Petra Karla Böckelmann , Renato Prado Costa ,
1,2
2
1
2
2
Carlos Hermann Schaal , Fernando César Sala , André Pereira Vanni ,
2
3
1,4
Leandro Luiz Lopes de Freitas , João Carlos Cardoso Alonso ,
Gabriela Cardoso de Arruda Camargo , Gabriela de Oliveira ,
1
1
5
Bianca Ribeiro de Souza , Athanase Billis , and Wagner José Fávaro *
1
3
1 Laboratory of Urogenital Carcinogenesis and Immunotherapy (LCURGIN), Universidade Estadual
de Campinas (UNICAMP), Campinas City, São Paulo State, Brazil
2 Department of Urology, Amaral Carvalho Hospital (HAC), Jaú City, São Paulo State, Brazil
3 Department of Pathology, Medical School, Universidade Estadual de Campinas (UNICAMP),
Campinas City, São Paulo State, Brazil
4 Department of Urology, Paulínia Municipal Hospital, Paulínia City, São Paulo State, Brazil
5 Department of Obstetrics and Gynecology, OVCARE, University of British Columbia, Vancouver,
British Columbia, Canada
Abstract
Bladder cancer (BC) stands as the second most common urinary tract malignancy.
*Corresponding author: Recent years have witnessed a growing interest in investigating energy metabolism to
Wagner José Fávaro help with better understanding the energy sources harnessed by tumor cells. The aims
(favarowj@unicamp.br)
of the present study are to feature and compare cell energy metabolism profiles among
Citation: Costa GP, different histological grades of non-muscle-invasive BC (NMIBC) by adjusting their
Böckelmann PK, Costa RP,
et al. Profiling energy metabolism bioenergetic cellular indexes based on the specific tumor types. Forty urinary bladder
in normal bladder tissue and tissue samples from patients both with and without a diagnosis of urothelial lesions
non-muscle-invasive bladder were collected. Subsequently, samples were categorized into four groups comprising
cancer cases of different
histological grades. Tumor Discov. ten samples each, namely: normal (no urothelial lesions) group, low-grade pTa group,
2024:3(1):2290. high-grade pTa group, and high-grade pT1 group. These tissue samples were examined
https://doi.org/10.36922/td.2290 by means of immunohistochemistry and Western blotting to assess proteins involved
Received: November 22, 2023 in cell energy metabolism. Based on the current findings, the normal and low-grade
Accepted: January 9, 2024 pTa groups presented clear preference for the oxidative phosphorylation pathway;
Published Online: March 19, 2024 consequently, they recorded high bioenergetic cellular index. On the other hand,
Copyright: © 2024 Author(s). both the high-grade pTa and pT1 groups presented proclivity towards the glycolytic
This is an Open-Access article pathway. These observations, mainly those associated with the bioenergetic cellular
distributed under the terms of the
Creative Commons Attribution index, hold promising clinical relevance in the management of BC. Given the often
License, permitting distribution, aggressive and potentially debilitating nature of treatments applied to this neoplasia
and reproduction in any medium, type, the current study offers invaluable insights on this topic and emphasizes changes
provided the original work is
properly cited. in the bioenergetic cellular index at different NMIBC grades, which could serve as
potential markers for both the diagnosis and prognosis of NMIBC patients.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Bladder cancer; Energy metabolism; Bioenergetic cellular index; Cancer
affiliations. metabolism

Volume 3 Issue 1 (2024) 1 https://doi.org/10.36922/td.2290