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Gene & Protein in Disease A pan-cancer analysis of HMGB1

A

B C

D

Figure 4. Tumor-associated protein phosphorylation of HMGB1. Comparison of HMGB1 phosphorylated protein levels (S35 and S100 sites) in normal
and selected primary tumor tissues. (A) Phosphorylated protein sites detected in HMGB1 based on the CPTAC dataset are shown in the figure. (B–D) Box
plots representation of HMGB1 phosphorylated protein levels in BRCA, UCEC and lung cancer.
HMGB1: High mobility group box 1; UCEC: Uterine corpus endometrial carcinoma; CPTAC: Clinical Proteomic Tumor Analysis Consortium;
BRCA: Breast cancer.

3.6. HMGB1 enrichment analysis ribonucleoprotein D0 (HNRNPD) (R=0.61), heterogeneous
To further research the molecular mechanism of HMGB1 nuclear ribonucleoprotein R (HNRNPR) (R = 0.63), KH
gene in the occurrence and development of tumor, the domain-containing, RNA-binding, signal transduction-
HMGB1-interacting proteins and HMGB1 expression- associated protein 1 (KHDRBS1) (R = 0.61), replication
related genes were screened by enrichment analyses. STRING factor C subunit 3 (RFC3) (R = 0.63), and structure-
was used to get a total of 50 HMGB1 interacting proteins specific recognition protein 1 (SSRP1) (R = 0.55) genes
which were verified in the experiments. The relationships (all P < 0.001, Figure 6B). The corresponding heatmap
of these proteins are displayed in Figure 6A. GEPIA2 was indicated that HMGB1 was positively correlated with the
applied to combine all tumor expression data of TCGA above six genes in most cancer types (Figure 6C). The
to obtain the top 100 genes that are related to HMGB1 interaction analysis of the above groups displayed that
expression. The expression level of HMGB1 was positively there were four common members, namely, SSRP1, SRSF1,
related to that of heterogeneous nuclear ribonucleoproteins SUPT16H, and high mobility group box 2 (HMGB2)
A2/B1 (HNRNPA2B1) (R = 0.68), heterogeneous nuclear (Figure 6D).

Volume 2 Issue 1 (2023) 7 https://doi.org/10.36922/gpd.301