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International Journal of Bioprinting 3D printed hydrogels for tumor therapy

For example, Yang et al. suggested that PDA particles MgHAp/GelMA and MgHAp/GelMA-PDA hydrogels
possessed a large amount of amine and hydroxyl groups significantly promoted osteogenic differentiation of
and thus benefited cell adhesion, spread, and growth. rBMSCs, which was consistent with previous studies. 36,71,72
68
2+
To investigate the osteogenic effect of printed Mg could regulate osteoblast activity by binding to
hydrogels, the ALP activity expression was studied. As the specific sites on the integrin α chains of cell surface
shown in Figure 12A and B, the ALP activity of rBMSCs and thus promote the expression of osteogenic genes.
2+
on HAp/GelMA hydrogels was higher than that on For example, Sun et al. showed that Mg released from
GelMA hydrogels. Previous studies have demonstrated Wollastonite ceramic could promote the ALP activity, the
that HAp nanocomposites could promote the osteogenic expression of osteogenic genes, such as Ocn, Runx2, Col1,
and Osx of MC3T3-E1 cells, and the repair of rabbit skull
differentiation of rBMSCs. 69,70 Moreover, MgHAp/GelMA defects. As a result, 3D-printed MgHAp/GelMA-PDA@
73
and MgHAp/GelMA-PDA hydrogels exhibited the highest DOX hydrogels showed great potential for treating bone
ALP activity expression after 7 and 14 days of culture, tumor-related defects by providing synergy for effectively
showing good osteogenic effect. Furthermore, the qRT- eradicating tumor cells and promoting osteogenesis.
PCR results, as shown in Figure 12C–F, indicated that
the expression of osteogenic genes, i.e., Ocn, Col1, Runx2, 4. Conclusion
and Bmp2, in MgHAp/GelMA and MgHAp/GelMA-PDA
hydrogels was upregulated in comparison to GelMA and In the current study, MgHAp nanocomposites were
HAp/GelMA hydrogels. The confocal images in Figure 12G synthesized via a biomineralization process. GelMA
further demonstrates that MgHAp/GelMA and MgHAp/ and PDA@DOX particles were fabricated. MgHAp/
GelMA-PDA hydrogels had the highest expression of GelMA-PDA@DOX hydrogels were constructed via 3D
COL1. As a result, the sustained release of Mg from printing. MgHAp nanocomposites and PDA particles
2+

Figure 11. Proliferation profiles of rBMSCs cultured on 3D-printed GelMA, HAp/GelMA, MgHAp/GelMA, and MgHAp/GelMA-PDA hydrogels.
** p < 0.01, *** p < 0.001 versus GelMA.

Volume 10 Issue 5 (2024) 250 doi: 10.36922/ijb.3526