Page 36 - IJB-7-3

3D Bioprinted Organoids
understanding of the molecular mechanisms of organ Development using Organoid Cultures. Development,
development, more bioprinting strategies for organoid 142:3113–25.
development will emerge. We believe that the future https://doi.org/10.1242/dev.118570
of bioprinting research will revolve around the studies 5. Lui JH, Hansen DV, Kriegstein AR, 2011, Development and
on early developmental stages of organs and tissues. Evolution of the Human Neocortex. Cell, 146:18–36.
Bioprinting organoid technology will potentially play
an extraordinary role in developmental biology, disease https://doi.org/10.1016/j.cell.2011.06.030
pathology, cell biology, regenerative mechanisms, 6. Kim J, Koo BK, Knoblich JA, 2020, Human Organoids:
precision medicine, and drug screening. Model Systems for Human Biology and Medicine. Nat Rev
Mol Cell Biol, 21:571–84.
6. Conclusion https://doi.org/10.1038/s41580-020-0259-3

Both bioprinting and organoids are intriguing research 7. Groll J, Boland T, Blunk T, et al., 2016, Biofabrication:
topics in the field of regenerative medicine. Bioprinting Reappraising the Definition of an Evolving Field.
emphasizes on the reconstrution of tissue structures, Biofabrication, 8:5.
while organoids fouces on the rebiulding of biological https://doi.org/10.1088/1758-5090/8/1/013001
functions. When these two technologies are combined,
bioprinted organs with both physiological function 8. Heinrich MA, Liu WJ, Jimenez A, et al., 2019, 3D Bioprinting:
and structure may come into reality in the near future. From Benches to Translational Applications. Small, 15:47.
Although organoid bioprinting is still in its infancy phase, 9. Tuan RS, Boland G, Tuli R, 2003, Adult Mesenchymal Stem
this technique has brought us one step closer to truly 3D Cells and Cell-Based Tissue Engineering. Arthritis Res Ther,
printing personalized organs. 5:32–45.
Acknowledgments 10. Xu T, Gregory CA, Molnar P, et al., 2006, Viability and
Electrophysiology of Neural Cell Structures Generated by the
This research was supported by the Key R and D Program Inkjet Printing Method. Biomaterials, 27:3580–8.
of the Ministry of Science and Technology of China https://doi.org/10.1016/j.biomaterials.2006.01.048
(No. 2018YFB1105600/2020YFC2008700/2018YFB11 11. Li X, Liu B, Pei B, et al., 2020, Inkjet Bioprinting of
07000), National Natural Science Foundation
of China (No. 92048205/81902195/82072228), Biomaterials. Chem Rev, 120:10793–833.
the Project of Shanghai Science and Technology 12. Xu C, Chai W, Yong H, et al., 2015, Scaffold-Free Inkjet
Commission (No. 19XD1434200), and Shanghai Printing of Three-Dimensional Zigzag Cellular Tubes.
Municipal Commission of Economy and Information Biotechnol Bioeng, 109:3152–60.
(No. 202001007). https://doi.org/10.1002/bit.24591

Conflict of interest 13. Lee YB, Polio S, Lee W, et al., 2010, Bio-Printing of Collagen
and VEGF-Releasing Fibrin Gel Scaffolds for Neural Stem
The authors declare that they have no conflicts of interest. Cell Culture. Exp Neurol, 223:645–52.

References https://doi.org/10.1016/j.expneurol.2010.02.014
14. Mandrycky C, Wang Z, Kim K, et al., 2016, 3D Bioprinting for
1. Fatehullah A, Tan SH, Barker N, 2016, Organoids as an Engineering Complex Tissues. Biotechnol Adv, 34:422–34.
in Vitro Model of Human Development and Disease. Nat Cell https://doi.org/10.1016/j.biotechadv.2015.12.011
Biol, 18:246–54. 15. Vijayavenkataraman S, Wei-Cheng Y, Lu WF, et al., 2018,
https://doi.org/10.1038/ncb3312 3D Bioprinting of Tissues and Organs for Regenerative
2. Matano M, Date S, Shimokawa M, et al., 2015, Modeling Medicine. Adv Drug Deliv Rev, 132:296–332.
Colorectal Cancer Using CRISPR-Cas9-Mediated https://doi.org/10.1016/j.addr.2018.07.004
Engineering of Human Intestinal Organoids. Nat Med, 16. Othon CM, Wu X, Anders JJ, et al., 2008, Single-Cell Printing
21:256–62. to form Three-Dimensional Lines of Olfactory Ensheathing
https://doi.org/10.1038/nm.3802 Cells. Biomed Mater, 3:034101.
3. Ballard DH, Boyer CJ, Alexander JS, 2019, Organoids- https://doi.org/10.1088/1748-6041/3/3/034101
Preclinical Models of Human Disease. N Engl J Med, 17. Pirlo RK, Wu P, Liu J, et al., 2011, PLGA/Hydrogel Biopapers
380:1981–2. as a Stackable Substrate for Printing HUVEC Networks Via
4. Huch M, Koo BK, 2015, Modeling Mouse and Human BioLP. Biotechnol Bioeng, 109:262–73.

32 International Journal of Bioprinting (2021)–Volume 7, Issue 3