Page 440 - IJB-9-2

International Journal of Bioprinting

RESEARCH ARTICLE
A perspective on light-based bioprinting of DNA

hydrogels for advanced bone regeneration:
Implication for bone organoids

Long Bai , Mengmeng Li , Jiacan Su*
†
†
Organoid Research Center, Institute of Translational Medicine, Shanghai University, Shanghai
200444, China
(This article belongs to the Special Issue: Advanced light-based bioprinting)

Abstract

Light-based three-dimensional (3D) printing of hydrogels has been widely adopted
for accelerating bone regeneration. However, the design principles of traditional
hydrogels do not take into consideration the biomimetic regulation of multiple
stages throughout the bone healing, and the hydrogels made cannot effectively
induce sufficient osteogenesis, which in turn greatly limits their capacity in guiding
bone regeneration. The recent progress achieved in DNA hydrogel, which is based
on synthetic biology, could facilitate the innovation of the current strategy due to
its advantages, such as resistance to enzymatic degradation, programmability,
structural controllability, and mechanical properties. However, 3D printing of DNA
hydrogel is not well defined and appears to have a few distinct early forms. In this
† These authors contributed equally article, a perspective on the early development of 3D printing of DNA hydrogels is
to this work.
presented, and a potential implication of the hydrogel-based bone organoids built-
*Corresponding author: up for bone regeneration is proposed.
Jiacan Su (drsujiacan@163.com)
Citation: Bai L, Li M, Su J, 2023,
A perspective on light-based Keywords: Three-dimensional printing; DNA hydrogel; Bone regeneration; Bone
bioprinting of DNA hydrogels for organoids; Tissue engineering
advanced bone regeneration:
Implication for bone organoids. Int J
Bioprint.
https://doi.org/10.18063/ijb.688
1. Introduction
Received: September 03, 2022
Accepted: October 31, 2022 Clinical treatment of bone defects caused by accidental bone trauma, bone tumors, and
Published Online: February 17,
[1]
2023 bone diseases remains challenging worldwide . The regeneration process of bone defect
is a complicated and sophisticated biological process that involves multiple interactions of
Copyright: © 2023 Author(s).
[2]
This is an Open Access article both cells and bioactive factors in three-dimensional (3D) extracellular matrix (ECM) .
distributed under the terms of the To properly mimic the 3D ECM environment that induces specific cell fate and
Creative Commons Attribution functions, a fabrication technology capable of precisely manipulating the 3D structure of
License, permitting distribution
and reproduction in any medium, the bone tissue as well as the spatial distribution of cells is required. Recent advances in
provided the original work is 3D printing have demonstrated the potential of the technology to fulfill the demands of
properly cited. controlling the location of biomaterials, living cells, and even growth factors to generate
[3]
Publisher’s Note: Whioce bioengineered structures . Over the years, light-based 3D bioprinting technology
Publishing remains neutral with has been widely adopted in medical devices due to its better pattern fidelity, spatial
regard to jurisdictional claims in
published maps and institutional resolution, and high efficiency in manufacturing processes. In particular, digital light
affiliations. processing (DLP)-based 3D printing has been gaining attention attributed to the high

Volume 9 Issue 2 (2023) 432 https://doi.org/10.18063/ijb.688