Page 446 - IJB-9-2

International Journal of Bioprinting

RESEARCH ARTICLE
A biocompatible double-crosslinked gelatin/

sodium alginate/dopamine/quaterniazed
chitosan hydrogel for wound dressings based on

3D bioprinting technology

Yueqi Lu , Jie Xu , Ya Su , Huan Fang , Jiaqi Liu , Siyao Lv , Yuen Yee Cheng ,
1
1
1
3
1,2
1,2
1,2
Yi Nie *, Wenfang Li *, Bo Pan *, Kedong Song *
5
1
2
4
1 State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering,
Dalian University of Technology, Dalian 116024, China
2 Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou 450000, China
3 Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology
Sydney, NSW 2007, Australia
4 School of Life Science and Technology, Weifang Medical University, Weifang, 261053, China
5 Department of Breast Surgery, The Second Hospital of Dalian Medical University, Dalian 116023,
*Corresponding authors: China
Kedong Song
(Kedongsong@dlut.edu.cn) (This article belong to the Special Issue: Novel Nanoencapsulation and Bio-Printing Technologies for Drug
Yi Nie Administration)
(ynie@ipe.ac.cn)
Wenfang Li Abstract
(liwenfang@wfmc.edu.cn)
Bo Pan Severe skin injuries can cause serious problems, which could affect the patient’s
(dmupanbo@hotmail.com)
normal life, if not dealt properly in a timely and effective manner. It is an urgent
Citation: Lu Y, Xu J, Su Y, et al., requirement to develop personalized wound dressings with excellent antibacterial
2023, A biocompatible double-
crosslinked gelatin/sodium alginate/ activity and biocompatibility to match the shape of the wound to facilitate clinical
dopamine/quaterniazed chitosan application. In this study, a bioink (GAQ) based on gelatin (Gel)/sodium alginate (SA)/
hydrogel for wound dressings based quaternized chitosan (QCS) was prepared, and GAQ hydrogel dressing grafting with
on 3D bioprinting technology. Int J
Bioprint. dopamine (GADQ) was fabricated by an extrusion three-dimensional (3D) printing
https://doi.org/10.18063/ijb.689 technology. QCS was synthesized by modifying quaternary ammonium group
on chitosan, and its structure was successfully characterized by nuclear magnetic
Received: October 25, 2022
Accepted: December 15, 2022 resonance (1H NMR) and Fourier-transform infrared spectroscopy (FT-IR). Our results
Published Online: February 20, showed that the GADQ hydrogel dressing that was double-crosslinked by EDC/
2023 NHS and Ca had good tensile strength, considerable swelling ratio, and effective
2+
Copyright: © 2023 Author(s). antioxidation properties. It also showed that GADQ1.5% had 93.17% and 91.06%
This is an Open Access article antibacterial activity against Staphylococcus aureus and Escherichia coli, respectively.
distributed under the terms of the
Creative Commons Attribution Furthermore, the relative survival ratios of fibroblast cells seeded on these hydrogels
License, permitting distribution exceeded 350% after cultured for 7 days, which proved the biocompatibility of these
and reproduction in any medium, hydrogels. Overall, this advanced 3D-printed GADQ1.5% hydrogels with effective
provided the original work is
properly cited. antioxidation, excellent antibacterial activity and good biocompatibility had a
considerable application potential for wound healing.
Publisher’s Note: Whioce
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Quaternized chitosan; 3D bioprinting; Dopamine; Hybrid scaffold;
affiliations. Wound dressings

Volume 9 Issue 2 (2023) 438 https://doi.org/10.18063/ijb.689