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International Journal of Bioprinting                               Multi-material bioprinting with OCT imaging




























































            Figure 11. Multi-layer scaffold design and printing results. (A) Designed path of three-layer scaffold. (B) OCT data projection of optimized three-layer
            scaffold and OCT data reconstruction map of the box area. (C) Printed three-layer scaffold before optimization. (D) Printed three-layer scaffold after
            optimization and actual layer thickness. (E) Layer thickness distribution at different locations in different layers of three-layer scaffold. (F) Total height at
            different locations in different layers of three-layer scaffold. (G) Designed path of nine-layer scaffold. (H) Printed nine-layer scaffold after optimization.
            (I) The OCT data reconstruction map of the same connection point during printing. I1 is before nozzle control optimization and I2 is after the optimization.
            (J) Total height in different layers of nine-layer scaffold.


            respectively, which indicates a good uniformity in layer   of their in vivo counterparts’ organs. The high-precision
            thickness between different materials.             printed structure is the basis of realizing the function of
                                                               target  structure.  However,  the  distinguished  properties
            4. Discussion                                      in  different  materials  lead  to  errors  in  multi-material
                                                               printing, which often requires a tedious trial-and-error
            As indicated by many studies, multi-material printing is   process to obtain a good outcome, resulting in a huge waste
            necessary for the bioprinted tissues to maintain the distinct   of material. Therefore, this study is aimed at establishing
            morphology features and complicated function signatures   a printing model (multi-material static model and time-


            Volume 9 Issue 3 (2023)                        251                          https://doi.org/10.18063/ijb.707
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