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International Journal of Bioprinting                           Comparison of different 3D printing technologies



            Table 4. Summary table of the difference of actual and target   Table 5. Summary table of the difference of actual and target
            values when performing the grid test               values when performing the grid test
                   Real value    Theoretical value   Standard         Real value    Theoretical value   Standard
                   (mm )         (mm )          deviation             (mm )        (mm )           deviation
                       2
                                                                                       2
                                                                         2
                                    2
             Row 1  0.42         2.55            1.51           Row 1  2.53         2.55            0.01
                    4.26         5.10            0.59                  5            5.10            0.07
                    8.05         10.20           1.52                 10.19         10.20           0.01
                   17.33         20.41           2.18                 20            20.41           0.29
             Row 2  4.48         5.10            0.44           Row 2  4.9          5.10            0.14
                    9.98         10.21           0.16                 10.12         10.21           0.06
                   17.52         20.41           2.04                 20.14         20.41           0.19
                   36.33         40.83           3.18                 40.9          40.83           0.05
             Row 3  6.50         7.65            0.81           Row 3  7.7          7.65            0.04
                   14.66         15.31           0.46                 15.25         15.31           0.04
                   25.78         30.62           3.42                 31            30.62           0.27
                   55.08         61.24           4.35                 62.2          62.24           0.03
             Row 4  8.96         10.20           0.88           Row 4  10           10.20           0.14
                   19.45         20.41           0.68                 20.44         20.41           0.02
                   36.23         40.83           3.26                 40.82         40.83           0.01
                   74.40         81.66           5.13                 81.62         81.66           0.03
             Row 5  10.36        12.76           1.69           Row 5  12.71        12.76           0.04
                   23.71         25.52           1.28                 25.55         25.56           0.01
                   48.80         51.03           1.58                 51            51.03           0.02
                   98.68         102.06          2.39                 102.02       102.06           0.03



            a great capacity to generate bridges with a high collapse   Table 6. Filament collapse test tested on the different hydrogels
            resistance. In addition, it showed a very good printability
            with no difference between the theoretical value and the   Material  C f 1  C f 2  C f 3  C f 4  C f 5  C f 6  C f 7  C f 8
            real value.                                         PCL      0   0    0    0   0    0    0   0

            3.3.1. Results of printing a biomimetic structure
            Good results, which were only obtained in the bioprinting
            of the gelatin-based hydrogel (GelMA) after following
            the printability methodology, show that the bioprinted
            structure  had  sufficiently  acceptable  structural
            characteristics for a complex structure. Before attempting
            to bioprint a biomimetic structure, the speed, temperature,
            and pressure parameters were adjusted to ensure the correct
            bioprinting of a simple scaffold with the different hydrogels
            created. Subsequently, using the Einscan-SE 3D scanner, a
            digital model of an example of a biomimetic structure, in
            this case a human tooth, was developed (Figure 9).
               As shown in Figure 9, the 5% GelMA hydrogel forms
            a tooth with a reliable shape with respect to the real tooth.
            After printing, a 405-nm ultraviolet light was applied for
            30 s to harden the hydrogel. The hardness of the generated
            structure was very low, as it was not possible to apply any
            pressure without causing a loss of its shape.      Figure 9. Bioprinting using 5% GelMA hydrogel. Source: own  elaboration.


            Volume 9 Issue3 (2023)                          34                         https://doi.org/10.18063/ijb.680
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