Page 395 - IJB-9-5

International Journal of Bioprinting

RESEARCH ARTICLE
A sturgeon cartilage extracellular

matrix-derived bioactive bioink for tissue
engineering applications

Xiaolin Meng , Zheng Zhou *, Xin Chen , Feng Ren *, Wenxiang Zhu ,
3
1
1
1
2
Shuai Zhu , Hairong Liu *
1
1
1 College of Materials Science and Engineering, Hunan University, Changsha 410082, PR China
2 College of Biology, Hunan University, Changsha 410082, PR China
3 Department of Geriatric Surgery, The Second Xiangya Hospital, Central South University,
Changsha, Hunan, 410011, PR China
(This article belongs to the Special Issue: Advances in 3D bioprinting for regenerative medicine and drug
screening)
Abstract

Three-dimensional (3D) bioprinting provides a promising strategy for tissue and
organ engineering, and extracellular matrix (ECM)-derived bioinks greatly facilitate
its applications in these areas. Decellularized sturgeon cartilage ECM (dSC-ECM)-
derived bioinks for cartilage tissue engineering were fabricated with methacrylate-
modified dSC-ECM (dSC-ECMMA) and sericin methacrylate (SerMA), which
optimizedthe mechanical properties of their solidified hydrogels.dSC-ECM induces
*Corresponding authors: chondrocytes to form cell clusters and subsequently reduces their proliferation,
Zheng Zhou but the proliferation of encapsulated chondrocytes was normal in solidified dSC-
(zhouzheng@hnu.edu.cn) ECM-5 bioink samples, which contain 5 mg/mL dSC-ECMMA. Hence, this bioink
Feng Ren was selected for further investigation. Lyophilized dSC-ECM-5 hydrogels showed
(renfeng@csu.edu.cn) connected pore microstructure, which is suitable for cell migration and nutrients
Hairong Liu
(liuhairong@hnu.edu.cn) transportation. ThisdSC-ECM-5 bioink exhibited high fidelity and good printability
by testing via a 3D bioprinting system, and the chondrocytes loaded in printed
Citation: Meng X, Zhou Z, Chen X,
et al., 2023, A sturgeon cartilage hydrogel products were viable and able to grow, following incubation, in the cell
extracellular matrix-derived bioactive culture medium. Solidified dSC-ECM-5 and SerMA bioinks loaded with chondrocytes
bioink for tissue engineering were subcutaneously implanted into nude mice for 4 weeks to test the suitability of
applications. Int J Bioprint, 9(5): 768.
https://doi.org/10.18063/ijb.768 the bioink for cartilage tissue engineering. Compared to the SerMA bioink, the dSC-
ECM-5 bioink significantly enhanced cartilage tissue regeneration and maturation
Received: March 28, 2023 in vivo, suggesting the potential of this bioink to be applied in cartilage tissue
Accepted: April 10, 2023
Published Online: June 6, 2023 engineering in the future.
Copyright: © 2023 Author(s).
This is an Open Access article Keywords: 3D bioprinting; ECM-derived bioink; Cartilage tissue engineering;
distributed under the terms of the
Creative Commons Attribution Cartilage regeneration
License, permitting distribution,
and reproduction in any medium,
provided the original work is
properly cited.
1. Introduction
Publisher’s Note: Whioce
Publishing remains neutral with The applications of 3D bioprinting allow people to manufacture living tissues and
regard to jurisdictional claims in organs in the future, and a practicable 3D bioprinting is a collection of science and
published maps and institutional [1,2]
affiliations. technologies . Theoretically, tissues and organs are composed of extracellular matrix

Volume 9 Issue 5 (2023) 387 https://doi.org/10.18063/ijb.768