Page 118 - IJB-9-6

International

Journal of Bioprinting

REVIEW ARTICLE
Exosome-based bioinks for 3D bioprinting

applications in tissue engineering and
regenerative medicine

Qian Wang , Yang Liu , Shuqing Zhang , Fan He , Tong Shi , Jizong Li *,
1†
1†
1
3
3
2
Zhimin Wang *, and Jia Jia *
1,2
4
1 School of Life Sciences, Shanghai University, Shanghai, China
2 Sino-Swiss Institute of Advanced Technology, School of Micro-electronics, Shanghai University,
Shanghai, China
3 Shanghai Center of Biomedicine Development, Shanghai, China
4 Shanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for
Biomedical and Pharmaceutical Technologies (SIBPT), Shanghai, China
(This article belongs to the Special Issue: 3D Printing for Tissue Engineering and Regenerative Medicine)
Abstract

Bioprinting is an emerging technology for tissue engineering and regenerative
medicine. Despite its fast, accurate manufacture for tissues and organs in vitro,
bioprinting has been seriously limited for biofabrication because of the restricted
approaches to reproducing the extracellular matrix (ECM) with sufficient bioactivities
† These authors contributed equally for bioprinted cells. Exosomes are natural biological particles with proteins, lipids,
to this work.
or genetic materials. They have distinct properties and unique biological functions
*Corresponding authors: to manipulate cellular behaviors and cell fates, showing great potential to support
Jizong Li (jizongli@stcsm.sh.gov.cn) cells for bioprinting. Here, we reviewed the recent progresses of exosome-advanced
Zhimin Wang
(wangzhm@chgc.sh.cn) bioprinting for tissue engineering and regenerative medicine. Firstly, we offer an
Jia Jia overview of the basics of exosomes and the current representative applications of
(jiajia748@126.com) exosomes in bone tissue engineering, immunological regulations, angiogenesis, and
neural regenerations. Then, a brief introduction about the bioinks and the currently
Citation: Wang Q, Liu Y, Zhang S,
et al., 2023, Exosome-based bioinks developed bioprinting methods is provided. We further give an in-depth review of the
for 3D bioprinting applications in biomedical applications of bioprinting with exosomes, majorly in bone engineering,
tissue engineering and regenerative vascular engineering, therapy of neuron injury, and skin regeneration. We also
medicine. Int J Bioprint, 9(6): 0114.
https://doi.org/10.36922/ijb.0114 conclude with an outlook on the challenges of the unmet needs of bioprinting cells
with correspondent ECM environments through bioprinting with exosomes.
Received: April 4, 2023
Accepted: May 12, 2023
Published Online: July 7, 2023
Keywords: Exosomes; Bioprinting; Bioinks; Regenerative medicine; Tissue
Copyright: © 2023 Author(s). engineering
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium, 1. Introduction
provided the original work is
properly cited. Bioprinting is derived from three-dimensional (3D) printing technology. It can apply
Publisher’s Note: AccScience cells, proteins, DNA, and other biological materials into personalized 3D models or
Publishing remains neutral with 3D biological functional structures through printing technology in vitro. Bioprinting
regard to jurisdictional claims in
published maps and institutional operated with computer-assisted designs (CADs) has exhibited the potential to
affiliations. reproduce the complexity of native tissues in terms of the mechanical properties, the

Volume 9 Issue 6 (2023) 110 https://doi.org/10.36922/ijb.0114