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International Journal of Bioprinting Affordable temperature-controlled bioprinter

projects. The ability to bioprint cell-laden constructs with Nacional de Ciencia y Tecnología, México) in the form
retained cell viability is also positively illustrated. of Graduate Program Scholarships. GTdS and MMA
This work demonstrates a method to more accurately acknowledge funding from the UC-MEXUS program.
control the rheology of 3D printer inks, such as GelMA GTdS acknowledges funding received from CONACyT
or Pluronic acid, whose viscosity is improved with and L’Oréal-UNESCO-CONACyT-AMC (National
temperature control. Moreover, the printing system Fellowship for Women in Science, México). MMA and
described here may be used as a simple platform to analyze GTdS acknowledge funding provided by CONACyT in the
the effect of different temperatures, flow rates, and feed form of Scholarships as members of the National System of
rates on fidelity and printability. Researchers (grant SNI 26048 and SNI 256730) and funding
provided by Saya Bio. The authors also acknowledge the
Future work should address the limitations and primary antibodies provided by Santa Cruz Biotechnology
opportunities provided by the current setup. The first Inc. in form of free sample.
concern involves the optimization of the thermal
conductivity of the printhead, and thermal analysis and Conflict of interest
simulations can be conducted to determine the most
efficient cooling/heating design. The authors have no conflicts of interest.
The integration of the syringe pump with the bioprinter Author contributions
(i.e., combining the motor and DiY printhead into one
component) should also be investigated, but for the Conceptualization: Mario Moisés Alvarez, Grissel Trujillo-
purposes of this work, we focused on the development of de Santiago, Andrés García-Rubio
a temperature controlling printhead. A sterile enclosure Formal analysis: Mario Moisés Alvarez
should also be designed and fixed to the bioprinter to Investigation: Carlos Ezio Garciamendez-Mijares, Gilberto
ensure sterile conditions for all bioprinting sessions, Emilio Guerra-Alvarez, Andrés García-Rubio, Mónica
especially when cells are used. These additions will be Gabriela Sánchez-Salazar, Anne-Sophie Mertgen,
very interesting contributions to the present system. Carlos Fernando Ceballos-González, Edna Johana
Nevertheless, our current findings show promising results Bolivar-Monsalve, German Garcia-Martinez
and should be readily reproducible by other laboratories Methodology: Mario Moisés Alvarez, Grissel Trujillo-
seeking affordable methods to make further progress in the de Santiago, Andrés García Rubio, Carlos Ezio
tissue engineering field. Garciamendez-Mijares, Gilberto Emilio Guerra-
Alvarez, Mónica Gabriela Sánchez-Salazar, German
Acknowledgments Garcia-Martinez
Writing—original draft: Mario Moisés Alvarez, Carlos Ezio
We gratefully acknowledge the experimental Garciamendez-Mijares
contributions of Alberto Emanuel Aceves Colin. The Writing—review & editing: Mario Moisés Alvarez, Yu
authors also acknowledge the primary antibodies Shrike Zhang, Grissel Trujillo-de Santiago
provided by Santa Cruz Biotechnology Inc. in form
of free samples. MGSS, CFCG, and EJBM gratefully Ethics approval and consent to participate
acknowledge financial support granted by CONACyT
(Consejo Nacional de Ciencia y Tecnología, México) Not applicable.
in the form of Graduate Program Scholarships. GTdS
and MMA acknowledge funding from the UC-MEXUS Consent for publication
program. GTdS acknowledges funding received from Not applicable.
CONACyT and L’Oréal-UNESCO-CONACyT-AMC
(National Fellowship for Women in Science, México). Availability of data
MMA and GTdS acknowledge funding provided by
CONACyT in the form of Scholarships as members of All date needed to reproduce the experiments described
the National System of Researchers (grant SNI 26048 and in this contribution have been made available in the main
SNI 256730) and funding provided by Saya Bio. body of the paper or in the Supplementary File.
Funding Further disclosure

MGSS, CFCG, and EJBM gratefully acknowledge Part of the entire set of findings has been presented in
financial support granted by CONACyT (Consejo AIChE Meeting, 2020.

Volume 9 Issue 6 (2023) 107 https://doi.org/10.36922/ijb.0244