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Gene & Protein in Disease DNA methylation and gene expression on rats with protein malnutrition

A B

C

Figure 14. Scatter plot of GO enrichment of methylation levels. (A) is for early-life low-protein group (LPE) versus control group (CON); (B) is for fetal
low-protein group (LPF) versus CON; and (C) is for LPF versus LPE.

expressed in small intestines, and its role is to catalyze the is PPPLR3D, a glycogen-targeting subunit of protein
synthesis of diacylglycerol (DAG) from free fatty acids phosphohydrolase 1 family, which is mainly distributed in
(FFA) and Sn-monoacylglycerol (MG) from dietary liver, skeletal muscle, pancreas, and brain. Recent studies
fats. Mgat2-deficient mice may delay FFA and Sn-MG have shown that the glycogen activity of PPPLR3D is
absorption from the proximal to distal parts of the small regulated by ubiquitination, and PPPLR3D is associated
intestine, increase energy consumption and regulate diet- with a specific phosphate-epileptic protein involved in
induced thermal energy production, and show resistance Lafora disease , a type of progressive myoclonic epilepsy.
[51]
to obesity, glucose tolerance, hypercholesterolemia, and In this study, the expression of Ppplr3d was downregulated
hepatic steatosis. However, in this study, the expression of in both LPE versus CON and LPF versus CON groups, so
Mgat2 was upregulated in both the LPF versus CON and it was speculated that this gene variation might also lead
LPF versus LPE groups, which means that the offspring to neuro-related diseases in early malnourished adult rats.
of early protein-malnourished rats may develop chronic
diseases, such as obesity and hepatic steatosis, due to This study found that Mt-nd (2, 3, 4L, 4, 5, 6, ATP-6,
the increased expression of Mgat2. Another example and ATP-8) and DNA methylation were differentially

Volume 1 Issue 2 (2022) 18 https://doi.org/10.36922/gpd.v1i2.169