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International Journal of Bioprinting Sr-doped printed scaffolds for bone repair

Figure 7. The effect of scaffolds on the osteogenic differentiation performance of BMSCs in vitro. (A1 and A2) ALP staining (A1) and ALP activity analysis
(A2) of P, SBP, and PSBP scaffolds co-cultured with BMSCs. (B) Expression of osteogenesis-related genes in the P, SBP, and PSBP scaffolds co-cultured
with BMSCs (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). Scale bar: 400 μm (A1). Abbreviations: BMSCs, bone marrow mesenchymal stem cells; OD, optical
density; P, polycaprolactone (PCL); PSBP, polydopamine (PDA)/strontium (Sr)-doped bioactive glass (SrBG)/polycaprolactone (PCL); SBP, strontium
(Sr)-doped bioactive glass (SrBG)/polycaprolactone (PCL).

SBP and P scaffolds, respectively; CD206 gene expression effect of the scaffolds, the levels of pro-inflammatory
in the PSBP scaffold was 6.5 and 1.6 times higher than factor IL-12 and anti-inflammatory factor IL-10 were
that in the SBP and P scaffolds, respectively; TNF-α gene detected by ELISA assay. Compared to the SBP scaffold,
expression in the P and SBP scaffolds was 2.3 and 2.6 the PSBP scaffold suppressed the expression level of pro-
times higher than that in the PSBP scaffold, respectively;
and IL1β gene expression in the P and SBP scaffolds was inflammatory factor IL-12 and increased the expression
12.3 and 14.2 times higher than that in the PSBP scaffold, level of anti-inflammatory factor IL-10 (p < 0.05)
respectively. To further verify the immunomodulatory (Figure 8B1 and B2).

Volume 11 Issue 4 (2025) 361 doi: 10.36922/IJB025210211