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Table 1. (Continued)
References Device application/research Device material/ Hydrodynamic Main cell type/ Key benefits and
objectives fabrication means mechanism microenvironment enhancements offered by
material the device
Aleman et al. 62 Deconstructed multi-niche PDMS/ Peristaltic pump h-BMNC; h-BMSC/ Co-culture in a perfused
microfluidic platform soft-lithography dithiolated 3D hydrogel matrix
modeling photocrosslinked
Dissecting normal and hydrogel Provides a structurally
malignant HSPC-niche deconvoluted and
interactions through a dynamically perfused
deconstructed multi-niche microenvironment
platform that integrates cell compared to conventional
tracking and recirculating animal models, improving
perfusion within a closed the quantification of normal
microfluidic system and malignant HSPC-niche
interactions with real-time
multi-niche spatial mapping
Ma et al. 63 Organotypic PDMS/ Reservoir h-BMSC; h-UVEC; Co-culture in a perfused
leukemia-on-a-chip soft-lithography human cord 3D hydrogel matrix
modeling blood CD34+cell;
Deciphering B-ALL h-BMNC; human Provides a patient-specific
chemoresistance through B-ALL cells/ and multi-niche leukemic
an organotypic chip that gelatin-fibrin microenvironment
replicates patient-derived, composite hydrogel compared to conventional
multi-niche leukemic homogeneous models,
microenvironments for improving the dissection
targeted therapy screening of heterogeneous
chemoresistance
mechanisms across B-ALL
subtypes with spatial-genetic
dual-resolution analysis
Chou et al. 64 Vascularized bone PDMS/ Peristaltic pump h-BMSC; h-HSPC; Co-culture in a perfused
marrow-on-a-chip with soft-lithography human-derived 3D hydrogel matrix
dual-channel architecture bone marrow
Recapitulating clinical bone mononuclear cell/ Provides a vascularized
marrow toxicities through a fibrin gel and patient-specific
dual-channel chip platform pathophysiological
that models patient-specific microenvironment
pathophysiology for rare compared to conventional
disease mechanism discovery non-accessible bone
marrow models,
improving the
recapitulation of clinical
myelotoxicity and recovery
dynamics with week-long
temporal resolution
Souquet et al. 65 Compartmentalized PDMS/maskless Data not available h-UVEC; h-FOB; Perfused 3D hydrogel
bone marrow-on-a-chip photolithography h-HSPC/collagen-I matrix
fabrication modeling and fibrin matrix
Deciphering niche-specific Provides a spatially
HSPC regulation through decoupled and
an optically accessible geometrically tunable
platform that mimics BMME compared to
vascular and endosteal niche conventional opaque
segregation with maskless bone models, improving
photolithography-engineered the understanding of
microarchitectures niche-specific HSPC
regulation with cellular
behavior tracking
capabilities
(Cont'd...)

Volume 1 Issue 3 (2025) 8 doi: 10.36922/OR025200017